Identification of Phenolic Dermal Sensitizers in a Wound Closure Tape

Details

• Personal Author:
  Anderson, Stacey E. ; Beezhold, Donald H. ; Butterworth LF ; Fedorowicz A ; Law, Brandon F. ; Meade BJ ; Myers LP ; Shapiro M ; Siegel, Paul D. ; Sussman G
• Description:
  A latex-allergic patient presented with a severe local reaction to a non-latex wound closure bandage following surgery. Extracts of the bandage were analyzed by gas chromatograph-electron impact-mass spectrometry (GC EI-MS) in the total ion monitoring mode. Components were identified by their ion mass fingerprint and elution time as a corresponding standard from the GC column. The chemicals identified were 4,4'-thiobis-(6-tert-butyl-m-cresol) (TBBC), 6-tert-Butyl-m-cresol (BC), 2,4-di-tert-butylphenol (BP) and erucamide (EA). Sensitization potential of these chemicals was evaluated using two quantitative structure-activity relationship (QSAR) programs. The phenol 2,6-di-tert-butyl-4-(hydroxymethyl)phenol (BHP) was also included in the test series. It was initially thought to be present in the bandage but detectable levels could not be confirmed. The potential for TBBC to induce a sensitization response was predicted by both Derek for Windows and TOPKAT 6.2. The potential for BC and BP to induce a sensitization response was predicted by Derek for Windows, but not TOPKAT. BHP and EA were not predicted to be sensitizers by either QSAR program. Local lymph node assay (LLNA) analysis of the chemicals identified TBBC, BP, and BC as potential sensitizers with EC3 values between 0.2 and 4.5%. None of the animals exhibited body weight loss or skin irritation at the concentrations tested. In agreement with the toxicological modeling, BHP did not induce a sensitization response in the LLNA. Following a positive LLNA response, TBBC, BP, and BC were further characterized by phenotypic analysis of the draining lymph nodes. A positive LLNA result coupled with a lack of increase in B220+IgE+ cell and serum IgE characterize these chemicals as Type IV sensitizers. These studies used a multidisciplinary approach combining clinical observation, GC-EI-MS for chemical identification, QSAR modeling of chemicals prior to animal testing, and the LLNA for determination of the sensitization potential of chemicals in a manufactured product. [Description provided by NIOSH]
• Subjects:
  Animals Chemistry Hypersensitivity Mass Spectrometry Occupational Health Phenol Safety
• Keywords:
  Animal-studies Chemical-analysis Gas-chromatography Mass-spectrometry Phenolic-compounds Phenols Sensitization Surgery
• ISSN:
  1547-691X
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  303-310
• Volume:
  4
• Issue:
  4
• NIOSHTIC Number:
  nn:20033372
• Citation:
  J Immunotoxicol 2007 Oct; 4(4):303-310
• Contact Point Address:
  Dr. Stacy E. Anderson, National Institute for Occupational Safety and Health (NIOSH), 1095 Willowdale Drive, Morgantown, WV 26505
• Email:
  sanderson4@cdc.gov
• Federal Fiscal Year:
  2008
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Immunotoxicology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:b6114a06164c794229578f2a7281d412495ae06646c71b13149789c543228384883fe16ca05f069cd6cd29681af6e92bb3823903a6c58ee6b8cb853edfefa74e
• Download URL:
  https://stacks.cdc.gov/view/cdc/188983/cdc_188983_DS1.pdf
• File Type:
  [PDF - 345.55 KB ]