A Rapid Most-Probable-Number-Based Enzyme-Linked Immunosorbent Assay for the Detection and Enumeration of Salmonella typhimurium in Poultry Wastewater

Details

• Personal Author:
  Goodridge CF ; Goodridge LD ; Gottfried D ; Wyvill JC
• Description:
  The rapid and accurate detection and enumeration of low levels of Salmonella Typhimurium in food processing facilities are critical components of an effective hazard analysis critical control point program. The objective of this study was to develop a rapid (8 h) most probable number (MPN)-enzyme-linked immunosorbent assay (ELISA) for the detection and enumeration of Salmonella Typhimurium in wastewater. The specific objectives were to (i) characterize poly- and monoclonal Salmonella Typhimurium-specific antibodies in order to select the most specific and sensitive antibody for Salmonella Typhimurium detection, and (ii) validate the MPN assay through a correlation between the 8-h MPN-ELISA and the traditional 48-h Salmonella Typhimurium MPN method in poultry scald water. Poultry scald water samples were spiked with 10 and 50 CFU/ml of Salmonella Typhimurium. The traditional MPN method used a 48-h enrichment period followed by an analysis, while the MPN-ELISA used a 5-h enrichment period followed by a 3-h ELISA analysis. No differences (P < 0.05) were found between the traditional MPN and the MPN-ELISA, indicating the promise of the MPN-ELISA for the rapid detection and enumeration of Salmonella Typhimurium within an 8-h shift. This abbreviated assay will permit increased product sampling and more rapid movement of food between production and processing, resulting in reduced spoilage and quality losses. [Description provided by NIOSH]
• Subjects:
  Bacterial Infections Ecology Education Environment Environmental Exposure Environmental Health Environmental Pollution Food-Processing Industry Food Contamination Food Handling Food Industry Hazardous Substances Livestock Manufacturing And Industrial Facilities Microbiology Occupational Health Risk Assessment Risk Factors Safety Safety Management Stress, Psychological Workplace

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• Keywords:
  Bacteria Bacterial-cultures Bacterial-disease Bacterial-infections Bactericides Bacteriology Contaminated-food Ecological-systems Environmental-contamination Environmental-exposure Environmental-factors Environmental-hazards Environmental-health Environmental-pollution Environmental-protection Environmental-stress Factory-workers Food Food-contaminants Food-handlers Food-processing Food-processing-equipment Food-processing-industry Food-processing-workers Food-services Food-spoilage Health-hazards Health-standards Microbial-test-systems Microchemistry Microorganisms Microscopic-analysis Microsomal-enzymes Pathogenesis Pathogenicity Pathogens Risk-analysis Risk-factors Safety-climate Safety-education Safety-equipment Safety-measures Safety-monitoring Safety-personnel Safety-practices Safety-programs Samplers Sampling Slaughterhouses Work-environment Work-practices Worker-health Workers Workplace-monitoring Workplace-studies

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• ISSN:
  0362-028X
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  Colorado ; Georgia ; OSHA Region 4 ; OSHA Region 8
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  66
• Issue:
  12
• NIOSHTIC Number:
  nn:20035330
• Citation:
  J Food Prot 2003 Dec; 66(12):2302-2306
• Contact Point Address:
  C. Goodridge, Electro-Optics, Environment and Materials Laboratory, Georgia Tech Research Institute, Atlanta, Georgia 30332-0823
• Email:
  cgoodrid@uwyo.edu
• Federal Fiscal Year:
  2004
• Performing Organization:
  Colorado State University - Fort Collins
• Peer Reviewed:
  True
• Start Date:
  20010930
• Source Full Name:
  Journal of Food Protection
• End Date:
  20030929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:ba47c2c44f1599522cb7688a30d7d8f67bd282b9ab3a83a46c5bb3dd36eda00eb290dc6d60f79252c291e9dde3b7d072614b4919bc59e275321477ecd19199e0
• Download URL:
  https://stacks.cdc.gov/view/cdc/187233/cdc_187233_DS1.pdf
• File Type:
  [PDF - 151.75 KB ]