Comparative Proteomics, Genomics and Pulmonary Toxicity of Instilled Single Walled Carbon Nanotubes, Crocidolite Asbestos and Ultrafine Carbon Black in Mice

Details

• Personal Author:
  Jacobs J ; Kisin E ; Murray A ; Pounds J ; Shvedova A ; Teeguarden J ; Varnum S ; Waters K ; Webb-Robertson B ; Zangar R
• Description:
  CNT have emerged as a nanomaterial of considerable concern to the occupational and environmental health and safety communities. Early, consistent reports from high-dose, short-term screening studies in rodents suggest a sequence of biological events and pulmonary pathology similar to that caused by asbestos and synthetic vitreous fibers (SVF). Whether CNT strictly adhere to the asbestos paradigm of pulmonary toxicity is a question of critical importance. The pulmonary response of mice exposed repeatedly (4 doses, 28 days, pharyngeal aspiration) to single-walled CNT (SWCNT), crocidolite asbestos (AS) and ultrafine carbon black (CB) was assessed by global proteomics and genomics of lung tissue and ELISA protein microarray of bronchial lavage fluid. Three cytokines, IL-6 and IL-12 and Mip-1g were considerably higher in SWCNT and AS treated animals compared to CB. The T cell chemotactic protein MDC was present in high levels in SWCNT and AS groups, but not detectable in control or CB treated groups. Two sentences on similarities and differences via proteomics. Microarray analysis of lung tissue revealed over 3000 genes significantly changed by SWCNT exposure, compared to only 469 with AS and 71 with CB. Strikingly, the significant genes lists for CB and AS were complete subsets of the SWCNT data set, with both the number of genes and magnitude of gene changes being the highest in the SWCNT treatment group. [Description provided by NIOSH]
• Subjects:
  Biological Factors Cytology Dust Environmental Exposure Environmental Monitoring Environmental Pollution Fibrosis Hazardous Substances Irritants Laboratories Lung Lung Diseases Microbiology Occupational Health Oxidants Particulate Matter Respiratory System Respiratory Tract Diseases Safety

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• Keywords:
  Biological-effects Biological-factors Cell-biology Cellular-reactions Environmental-exposure Environmental-hazards Exposure-assessment Exposure-levels Exposure-methods Fibrogenicity Fibrous-bodies Fibrous-dusts Genotoxic-effects Health-hazards Inhalation-studies Laboratory-animals Lung-cells Lung-disorders Lung-irritants Microscopic-analysis Microscopy Nanotechnology Oxidative-metabolism Oxidative-processes Particle-aerodynamics Particulates Pulmonary-disorders Pulmonary-system-disorders Respiratory-irritants Respiratory-system-disorders Statistical-analysis Toxic-effects

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• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 10 ; OSHA Region 3 ; Washington ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  108
• Issue:
  1
• NIOSHTIC Number:
  nn:20035310
• Citation:
  Toxicologist 2009 Mar; 108(1):460
• CAS Registry Number:
  Asbestos (CAS RN 1332-21-4) ; Carbon (CAS RN 7440-44-0) ; Carbon black (CAS RN 1333-86-4) ; Crocidolite asbestos (CAS RN 12001-28-4)
• Federal Fiscal Year:
  2009
• NORA Priority Area:
  Construction ; Manufacturing
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 48th Annual Meeting and ToxExpo, March 15-19, 2009, Baltimore, Maryland
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:c2e74a4fa68b5a8b6324e3bdfdd3de470f9f3f62206568352f2f50765de06891ec85a0e0acbbefad7071ede2a32c5f0b9184de54839ef54f8bb38ebf0bd98e92
• Download URL:
  https://stacks.cdc.gov/view/cdc/187218/cdc_187218_DS1.pdf
• File Type:
  [PDF - 1.86 MB ]