The Production and Characterization of Monoclonal Antibodies Against Toluene Diisocyanate (TDI)-Conjugated Proteins

Details

• Personal Author:
  Beezhold, Donald H. ; Blanchere, Francoise M. ; Hettick JM ; Janotka E ; Ruwona TB ; Schmechel D ; Siegel, Paul D.
• Description:
  RATIONALE: Toluene diisocyanate (TDI) is a highly reactive chemical and one of the most commonly reported causes of occupational asthma. The production of TDI-specific monoclonal antibodies (mAb) will allow the development of standardized immunoassays for exposure assessment. METHODS: Mice were immunized with TDI-keyhole limpet hemocyanin (KLH) conjugates and hybridomas were screened by ELISA using TDI conjugated to human serum albumin. MAbs were characterized by western blot against various mono- and diisocyanates conjugated to different carrier proteins using carbamate or thiocarbamate linkages. RESULTS: A total of 35 mAbs were produced (25 IgG1, 8 IgG2a, 2 IgG2b). Several mAbs were found to be specific for 2,4-TDI-conjugated proteins and did not react with 2,6-TDI-, monoisocyanate- or isothiocyanate -conjugated proteins. Other mAbs cross-reacted with the above conjugates, methylene diphenyldiisocyanate- and hexamethylene diisocyanate protein conjugates. All mAb reactivities were carrier protein independent. CONCLUSIONS: The mAbs can differentiate between specific isocyanates in a carrier independent fashion and may be useful as hapten-specific reagents for immunoassay development. [Description provided by NIOSH]
• Subjects:
  Antibodies Antigens Biochemistry Biological Factors Immune System Laboratories Occupational Health Respiratory System Safety
• Keywords:
  Antibody-response Biochemical-analysis Biological-effects Biological-factors Biological-systems Breathing Humans Immune-reaction Immunochemistry Laboratory-animals Laboratory-testing

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• ISSN:
  0091-6749
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  123
• Issue:
  2
• NIOSHTIC Number:
  nn:20035093
• Citation:
  J Allergy Clin Immunol 2009 Feb; 123(2)(Suppl 1):S150
• Email:
  zvf9@cdc.gov
• CAS Registry Number:
  Toluene 2,4-diisocyanate (CAS RN 584-84-9)
• Federal Fiscal Year:
  2009
• NORA Priority Area:
  Healthcare and Social Assistance ; Services
• Peer Reviewed:
  False
• Source Full Name:
  Journal of Allergy and Clinical Immunology
• Supplement:
  1
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:f060590e89fc9ed42a8908022fd9e84414a97a7f12d688f6a8a5833475229c933d8aa763c3b4c1bc0dc7c39ae4c455f824a208f44350b3230a179da3c4cbce97
• Download URL:
  https://stacks.cdc.gov/view/cdc/187062/cdc_187062_DS1.pdf
• File Type:
  [PDF - 45.03 KB ]