Magnetic Electrochemical Immunoassays with Quantum Dot Labels for Detection of Phosphorylated Acetylcholinesterase in Plasma

Details

• Personal Author:
  Lin Y ; Timchalk C ; Wang H ; Wang J
• Description:
  A new magnetic electrochemical immunoassay has been developed as a tool for biomonitoring exposures to organophosphate (OP) compounds, e.g., insecticides and chemical nerve agents, by directly detecting organophosphorylated acetylcholinesterase (OP-AChE). This immunoassay uniquely incorporates highly efficient magnetic separation with ultrasensitive square wave voltammetry (SWV) analysis with quantum dots (QDs) as labels. A pair of antibodies was used to achieve the specific recognition of OP-AChE that was prepared with paraoxon as an OP model agent. Antiphosphoserine polyclonal antibodies were anchored on amorphous magnetic particles preferably chosen to capture OP-AChE from the sample matrixes by binding their phosphoserine moieties that were exposed through unfolding the protein adducts. This was validated by electrochemical examinations and enzyme-linked immunosorbent assays. Furthermore, antihuman AChE monoclonal antibodies were labeled with cadmium-source QDs to selectively recognize the captured OP-AChE, as characterized by transmission electron microscopy. The subsequent electrochemical SWV analysis of the cadmium component released by acid from the coupled QDs was conducted on disposable screen-printed electrodes. Experimental results indicated that the SWV-based immunoassays could yield a linear response over a broad concentration range of 0.3-300 ng/mL OP-AChE in human plasma with a detection limit of 0.15 ng/mL. Such a novel electrochemical immunoassay holds great promise as a simple, selective, sensitive, and field-deployable tool for the effective biomonitoring and diagnosis of potential exposures to nerve agents and pesticides. [Description provided by NIOSH]
• Subjects:
  Agriculture Biochemistry Biological Factors Chemistry Chemistry, Analytic Environmental Exposure Environmental Health Environmental Monitoring Environmental Pollution Immune System Insecticides Occupational Health Particulate Matter Safety

  More +
• Keywords:
  Agricultural-chemicals Air-sampling Analytical-chemistry Biochemical-analysis Biological-effects Biological-factors Biological-monitoring Electrochemical-analysis Electrochemical-properties Electrochemical-reactions Environmental-contamination Environmental-exposure Environmental-factors Environmental-hazards Environmental-technology Immune-reaction Immunochemistry Immunotoxins Insecticide-poisoning Particle-aerodynamics Particle-counters Particulate-sampling-methods Pesticides Pesticides-and-agricultural-chemicals Quantitative-analysis

  More +
• ISSN:
  0003-2700
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 10 ; Washington
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  80
• Issue:
  22
• NIOSHTIC Number:
  nn:20035004
• Citation:
  Anal Chem 2008 Nov; 80(22):8477-8484
• Contact Point Address:
  Yuehe Lin, Pacific Northwest National Laboratory, Richland, Washington 99352
• Email:
  yuehe.lin@pnl.gov
• Federal Fiscal Year:
  2009
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  Battelle Pacific Northwest Laboratories
• Peer Reviewed:
  True
• Start Date:
  20060901
• Source Full Name:
  Analytical Chemistry
• End Date:
  20170831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:4c782d00b97d4a6377cc52fa3497d6d6e58439930b8b65dd930ff2a25ed49a83846e98d8575d4a4587e0b1b5a4dbee30ec7f20d8fd4719a90450e99447e42eca
• Download URL:
  https://stacks.cdc.gov/view/cdc/187007/cdc_187007_DS1.pdf
• File Type:
  [PDF - 1.54 MB ]