Inflammatory Properties of Iron-Containing Carbon Nanoparticles

Details

• Personal Author:
  Dutta PK ; Knight DA ; Kristovich R ; Waldman WJ
• Description:
  Inflammatory responses following exposure of carbon nanoparticles to human macrophage and endothelial cells were employed as indicators of particulate biological activity. Hundred nanometer carbon particles (nC) with and without nonextractable surface-bound iron were synthesized using a templating approach, and human monocyte-derived macrophages (MDM) were exposed to various concentrations of these particulates. Supernatants recovered from MDM 24 h postexposure were assayed for the inflammatory cytokine tumor necrosis factor-alpha (TNFalpha) by a quantitative ELISA and tested for their ability to induce expression of intercellular adhesion molecule-1 (ICAM-1) on human endothelial cells (EC) by immunofluorescence flow cytometry. Data generated by these experiments demonstrated that nC-Fe was far more biologically active than nC. In addition, the chemical reactivity of nC-Fe toward decomposition of hydrogen peroxide to form hydroxyl radicals was significantly higher than that of nC and correlated well with the increase in the strength of the inflammatory response, though a direct proof of creation of hydroxyl radicals in the biological system is not provided. Comparison with micrometer-sized carbon and carbon-iron particles suggests that the chemical and biological reactivity is correlated with surface area. [Description provided by NIOSH]
• Subjects:
  Aerosols Cells, Cultured Ferrous Compounds Occupational Health Particulate Matter Safety
• Keywords:
  Aerosol-particles Cell-cultures Cell-function Cellular-function Iron-compounds Nanotechnology Particulate-dust Particulates
• ISSN:
  0893-228X
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  20
• Issue:
  8
• NIOSHTIC Number:
  nn:20032938
• Citation:
  Chem Res Toxicol 2007 Aug; 20(8):1149-1154
• Contact Point Address:
  James Waldman, Department of Pathology, The Ohio State University College of Medicine, 4160 Graves Hall, 333 West 10th Avenue, Columbus, OH 43210
• Email:
  james.waldman@osumc.edu
• CAS Registry Number:
  Iron (CAS RN 7439-89-6)
• Federal Fiscal Year:
  2007
• Performing Organization:
  The Ohio State University
• Peer Reviewed:
  True
• Start Date:
  20060801
• Source Full Name:
  Chemical Research in Toxicology
• End Date:
  20100731
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:be29f6713e1e999379f8288cf1151c915278797fae980766fb7435fd7bdc87c6da9e93bf00853e93b506ae2859d577dbe53d1973615e8b1dcc3977cee44678be
• Download URL:
  https://stacks.cdc.gov/view/cdc/186718/cdc_186718_DS1.pdf
• File Type:
  [PDF - 475.53 KB ]