Aryl Radicals from Arenediazonium Ions Induce AP-1 In Vitro and In Vivo

Details

• Personal Author:
  Daft J ; Gannett PM ; Powell J ; Shi ZL ; Toth B ; Ye JP ; Daft J ; Gannett PM ; Powell J ; Shi ZL ; Toth B ; Ye JP
• Description:
  Agaricus bisporus the mushroom of commerce, contains genotoxic arylhydrazines and arenediazonium ions. In vitro, in cellular systems, and in vivo, arylhydrazines which are metabolically converted to arenediazonium ions and aryl radicals cause DNA damage that correlates with arylhydrazine or arenediazonium ion genotoxicity. The biochemical processes which connect the arenediazonium ion and genotoxicity has not been elucidated. We show here that arenediazonium ions induce AP-I (activator protein) in a dose-dependent manner in murine epidermal JB6p* cells. Co-treatment with the antioxidant N-acetylcysteine (NAC) inhibits AP-I induction and ESR spin-trapped aryl radicals. Acetylsalicylic acid (aspirin), however, does not induce AP-I nor does it quench aryl radical formation. In transgenic mice, selective AP-I induction was observed in the lung, a target tissue. Thus, activation of AP-l, caused by DNA damage and aryl radicals, may be involved in arylhydrazine genotoxicity. [Description provided by NIOSH]
• Subjects:
  Biochemistry Biological Factors Occupational Health Safety
• Keywords:
  Biological-effects Biological-factors Biological-function Biological-systems Cell-biology Cell-function Cell-metabolism Molecular-biology
• ISSN:
  0892-6638
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Proceedings ; Abstract or Summary ; Proceedings
• Place as Subject:
  Nebraska ; OSHA Region 3 ; OSHA Region 7 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH) ; National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division ; HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health ; Workplace Safety & Health
• Location:
  North America ; North America
• Volume:
  14
• Issue:
  4
• NIOSHTIC Number:
  nn:20032742
• Citation:
  FASEB J 2000 Mar; 14(4):A447
• Federal Fiscal Year:
  2000
• Peer Reviewed:
  True
• Source Full Name:
  The FASEB Journal
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:603234bfa279b66da891b74a9e3833c452ccd9505610fe321fcbf9394509af63498ad86545b189627617082baa07a5a9bd5a0184d02ef69e1326954b08295a24
• Download URL:
  https://stacks.cdc.gov/view/cdc/186599/cdc_186599_DS1.pdf
• File Type:
  [PDF - 210.16 KB ]