Factors in Standardizing Automated Cholinesterase Assays

Details

• Personal Author:
  Brimijoin S ; Dass PD ; Elliot G ; Henderson JD ; Jaeger B ; Lanz D ; Padilla S ; Pearson R ; Spies R ; Wilson BW
• Description:
  A scientific panel assembled by the U.S. Environmental Protection Agency (EPA) determined that variability in cholinesterase (ChE) activities in the agency's pesticide/animal study database likely was due to a lack of accepted guidelines for ChE methodology. A series of trials was held in which participating laboratories measured ChE activity in blood and brain samples from untreated and pesticide-treated rats using a colorimetric assay method. The degree of inhibition of ChE activity in plasma and brain samples compared to controls was consistent among most of the laboratories. The ChE activity in erythrocyte samples differed more between laboratories due to a high blank, low erythrocyte AChE activity and hemoglobin absorption at the wavelength of the assay. Strategies are suggested for minimizing the variability of ChE activity in hemoglobin-rich samples. [Description provided by NIOSH]
• Subjects:
  Chemistry Colorimetry Hematologic Tests Insecticides Occupational Health Safety
• Keywords:
  Analytical-instruments Analytical-methods Analytical-processes Blood-analysis Chemical-analysis Erythrocytes Pesticides Pesticides-and-agricultural-chemicals Sampling Sampling-methods
• ISSN:
  1528-7394
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  California ; Delaware ; Kansas ; Maryland ; Minnesota ; New Jersey ; OSHA Region 10 ; OSHA Region 2 ; OSHA Region 3 ; OSHA Region 5 ; OSHA Region 7 ; OSHA Region 9 ; Virginia ; Washington
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  187-195
• Volume:
  48
• Issue:
  2
• NIOSHTIC Number:
  nn:20032546
• Citation:
  J Toxicol Environ Health A 1996 Jun; 48(2):187-195
• Contact Point Address:
  Barry W. Wilson, Departments of Environmental Toxicology and Avian Sciences, University of California, Davis, California, USA
• Federal Fiscal Year:
  1996
• Performing Organization:
  University of California - Davis
• Peer Reviewed:
  True
• Start Date:
  19900930
• Source Full Name:
  Journal of Toxicology and Environmental Health, Part A: Current Issues
• End Date:
  20020929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:600399df15c7de4506a27cf9de605f860ecc82cc511938ddbab9256a94d28a81d9850b8743d0113beec9e7fcf3c71127afd1cbd36f082e97c82c244247645865
• Download URL:
  https://stacks.cdc.gov/view/cdc/186477/cdc_186477_DS1.pdf
• File Type:
  [PDF - 321.75 KB ]