Mechanisms of Skeletal Muscle Injury and Repair Revealed by Gene Expression Studies in Mouse Models

Details

• Personal Author:
  Chapman R ; Gao X ; Hulderman T ; Simeonova PP ; Summan M ; Warren GL
• Description:
  Common acute injuries to skeletal muscle can lead to significant pain and disability. The current therapeutic approaches for treating muscle injuries are dependent on the clinical severity but not on the type of injury. In the present studies, the pathophysiology and molecular pathways associated with two different types of skeletal muscle injury, one induced by direct destruction of muscle tissue (i.e. FI) and the other induced by a contractile overload (more specifically high-force eccentric contractions, i.e. CI) were compared side by side. Histopathological evaluation and measurements of muscle strength were accompanied by analyses of expression for 12 488 known genes at four time points ranging from 6 h to 7 days after injury. Real-time RT-PCR was used to confirm some of the injury type differences in the temporal profiles of gene expression. Our data revealed several pools of genes, including early induction of transcription, myogenic and stress-responsive factors, common for both types of injury as well as pools of genes expressed specifically with one of the injury types. Only CI activated a set of genes associated with the repair of impaired proteins and structures including genes related to apoptosis, whereas FI uniquely activated gene sets involved in extensive inflammatory responses, tissue remodelling, angiogenesis and myofibre/extracellular matrix synthesis. In conclusion, knowledge of the sets of genes associated specifically with the nature of the injury may have application for development of new strategies for acceleration of the recovery process in injured skeletal muscle. [Description provided by NIOSH]
• Subjects:
  Animals Genetics Musculoskeletal Diseases Musculoskeletal System Occupational Health Safety
• Keywords:
  Animal-studies Genes Genetic-factors Motion-studies Muscles Muscular-disorders Musculoskeletal-system Musculoskeletal-system-disorders Skeletal-movement Skeletal-system Skeletal-system-disorders Tissue-disorders

  More +
• ISSN:
  0022-3751
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Georgia ; OSHA Region 3 ; OSHA Region 4 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  582
• Issue:
  2
• NIOSHTIC Number:
  nn:20032533
• Citation:
  J Physiol 2007 Jul; 582(2):825-841
• Contact Point Address:
  P. P. Simeonova: National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505
• Email:
  psimeonova@cdc.gov
• Federal Fiscal Year:
  2007
• NORA Priority Area:
  Construction
• Peer Reviewed:
  True
• Source Full Name:
  The Journal of Physiology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:8098b4f01e8c8275ffd547c1edea4c9ab22cdf80a01409c4bc0555a9193d73c4456ecd94c716cfad5d0acf77a86ee724d4c278f0df77c6c914aef5564db236f3
• Download URL:
  https://stacks.cdc.gov/view/cdc/186470/cdc_186470_DS1.pdf
• File Type:
  [PDF - 1.13 MB ]