Development of Sensitive Immunoassays for the Detection of the Glucuronide Conjugate of 3-Phenoxybenzyl Alcohol, a Putative Human Urinary Biomarker for Pyrethroid Exposure

Details

• Personal Author:
  Ahn C ; Gee JS ; Hammock BD ; Kim JH ; Ma JS
• Description:
  Pyrethroids are widely used in agriculture as insecticides. This study describes a sensitive enzyme-linked immunosorbent assay for the detection of the glucuronide conjugate of 3-phenoxybenzyl alcohol, a putative pyrethroid metabolite that may be used as a biomarker of exposure to pyrethroids. Four antisera were elicited against two different immunizing haptens. Antisera were characterized in combination with several coating haptens. The lowest IC50 value (0.5 ng/mL) was obtained with antiserum 1891 and 3-phenoxybenzoic acid-BSA conjugate as the coating antigen. Antiserum 1891 was highly selective for the target compound with an overall cross-reactivity of <0.3% to structurally related compounds. The assay sensitivity was negligibly affected by pH 4-9. A 5-fold improvement in IC50 was observed using a 10-fold concentrated phosphate-fuffered saline as the assay buffer. Compared to assays conducted in normal phosphate-fuffered saline, the maximal absorbance was almost identical. A good correlation (r 2 = 0.99 and 0.97 for urine samples A and B, respectively) was observed between spiked levels and the levels detected by the immunoassay. [Description provided by NIOSH]
• Subjects:
  Agriculture Chemistry Enzymes Hypersensitivity Insecticides Occupational Health Safety Urine
• Keywords:
  Agricultural-chemicals Agricultural-industry Chemical-composition Chemical-hypersensitivity Chemical-reactions Enzymatic-disorders Enzymatic-effects Exposure-levels Exposure-limits Sensitization Urinalysis

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• ISSN:
  0021-8561
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  55
• Issue:
  10
• NIOSHTIC Number:
  nn:20032364
• Citation:
  J Agric Food Chem 2007 May; 55(10):3750-3757
• Contact Point Address:
  Department of Entomology and UCD Cancer Research Center, University of California, Davis, California 95616
• Federal Fiscal Year:
  2007
• Performing Organization:
  University of California - Davis
• Peer Reviewed:
  True
• Start Date:
  20010930
• Source Full Name:
  Journal of Agricultural and Food Chemistry
• End Date:
  20270929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:318fed956e8d8d1ce0021b8bc5036ec25ed745a71c0cbf3980bdd4e0deab326b663d795cd07fa6cb56840ab3965c6c18e98d2a74c80125d484a9541d3cbd5827
• Download URL:
  https://stacks.cdc.gov/view/cdc/186374/cdc_186374_DS1.pdf
• File Type:
  [PDF - 146.60 KB ]