Molecular and Morphologic Determinanats of Organic Dust Exposures in Alveolar Macrophage (AM) Cells

Details

• Personal Author:
  Beard ML ; Cosma GN ; Davis WF ; Olenchok SA ; Ufferfilge JE
• Description:
  To understand the pathology of pulmonary diseases after exposures to airborne dusts, we have previously measured acute-phase gene induction in rat/mouse lung after inhalation of respirable particulates. We have now measured in vitro responses of target AM cells to grain dust (GD) extracts and specific etiologic agents (bacterial endotoxin and fungal mycotoxins) found in elevator wheat samples. Area samples of respirable wheat dust were collected onto filters by personal pumps during routine elevator operations. Endotoxin levels were, quantitated by chromogenic LAL assay, and fungi identified by standard mycologic culture. Rat AM cell line NR8383 displayed greatly reduced cell growth and elevated toxicity in response to purified endotoxin vs. Alternaria mycotoxins. When cells were exposed to G D extracts, dose-responses most closely resembled those of endotoxin alone, suggesting a pivotal role for this febrile agent in the pathophysiology of dust-related pulmonary diseases, induction of the metallothionein gene was also detected by Northern blot analyses. Further acute-phase gene induction and oxidative burst analyses are in progress. [Description provided by NIOSH]
• Subjects:
  Air Pollutants, Occupational Animals Bacteria Dust Fungi Laboratories Lung Lung Diseases Occupational Health Particulate Matter Respiratory System Respiratory Tract Diseases Safety

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• Keywords:
  Airborne-particles Animal-studies Bacterial-dusts Dust-exposure Dust-particles Laboratory-animals Lung-disorders Lung-irritants Organic-dusts Particulate-dust Pulmonary-system-disorders Respirable-dust Respiratory-system-disorders

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• ISSN:
  0731-9193
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Abstract or Summary
• Place as Subject:
  Colorado ; OSHA Region 3 ; OSHA Region 8 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  96
• Volume:
  15
• Issue:
  1
• NIOSHTIC Number:
  nn:20032268
• Citation:
  Toxicologist 1995 Mar; 15(1):96
• Federal Fiscal Year:
  1995
• Performing Organization:
  Colorado State University
• Peer Reviewed:
  False
• Start Date:
  19910930
• Source Full Name:
  The Toxicologist. Society of Toxicology 34th Annual Meeting, March 5-9,1995, Baltimore, Maryland
• End Date:
  20020929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:8bab0a0fc81fa9d6f2b18fcafb7bdb6f41168b79650e9a0cf3a2b248ba8f6fdc255129579813b7a20fe3f9d4bd484ba400ca8e029584c7ea780196d69b525545
• Download URL:
  https://stacks.cdc.gov/view/cdc/186299/cdc_186299_DS1.pdf
• File Type:
  [PDF - 938.07 KB ]