In Vitro Exposure of Human Uroepithelial Cells to Occupational Chemical Results in Alterations in Protein Expression as Determined by High Resolution Two Dimensional Polyacrylamide Gel Electrophoresis

Details

• Personal Author:
  Athota K ; Frederickson S ; Kanitz MH ; Reznikoff C ; Savage RE ; Swaminathan S
• Description:
  A study has been initiated to investigate the carcinogenicity of 4,4-methylene bis(2-chloroaniline)(MOCA) and 2-phenyl-l,4-benzoquinone (PBQ) and to identify biomarkers of effects of exposure to these workplace-associated suspect carcinogens. High resolution two dimensional polyacrylamide gel electrophoresis (2D PAGE) was used to examine changes in protein expression in chemically-treated human uroepithelial cells (HUC). Previously, HUC has been shown to be sensitive to malignant transformation by 4-aminobiphenyl (4-ABP) and its metabolites (Bookland, et., at, Cancer Research, 52;160&1614, 1992). HUC was incubated for 24hr with the controls, dimethylsulfoxide (DMSO)or N-OH-AABP as well as N-OH-MOCA(2.5 and .10 uM), or PBQ (1.5.and 3.5 uM) which resulted in 25% and 75% cell kill, respectively. A 2D PAGE protein map of treated HUC was developed using the ISO-DALT method of Anderson. Electrophoretic pattern comparison across treatment groups by scanning densitometer and computer analysis revealed quantitative changes in a specific subset of proteins in the 10 uM MOCA-treated HUC and N-OH-AABP-treated HUC but not the DMSO-treated HUC. Preliminary studies of tumor incidence in athymic mice suggest that neo-plastic transformation occurred only in the 10 uM MOCA-treated HUC. These results show that occupational chemical exposure of HUC results in 2D PAGE protein alterations which may be useful for the identification of biomarkers associated with bladder tumorigenesis. [Description provided by NIOSH]
• Subjects:
  Animals Carcinogens Endocrine System Endocrine System Diseases Environmental Monitoring Laboratories Neoplasms Occupational Exposure Occupational Health Safety Urogenital Diseases Urogenital System

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• Keywords:
  Animal-studies Bladder-cancer Bladder-disorders Endocrine-system-disorders Exposure-assessment Exposure-limits Laboratory-animals Occupational-exposure Tumorigenesis
• ISSN:
  0731-9193
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  Ohio ; OSHA Region 5 ; Wisconsin
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DBBS - Division of Biomedical and Behavioral Sciences
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  201-202
• Volume:
  15
• Issue:
  1
• NIOSHTIC Number:
  nn:20032261
• Citation:
  Toxicologist 1995 Mar; 15(1):201-202
• Federal Fiscal Year:
  1995
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 34th Annual Meeting, March 5-9,1995, Baltimore, Maryland
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:0024872ff0edfc6efc81077aac87a4e3ab25cf57df6dfbaf20ec64e76d687d159d485a1cc5c6113fd902719eca815901b7790c2f405a5edec3876ba87e9e8767
• Download URL:
  https://stacks.cdc.gov/view/cdc/186292/cdc_186292_DS1.pdf
• File Type:
  [PDF - 1.70 MB ]