Interleukin 6 Plays a Critical Role in Cutaneous Wound Healing

Details

• Personal Author:
  Berry-Ann R ; Gallucci RM ; Guriel GK ; Kommineni C ; Luster MI ; Matheson JM ; Simeonova PP ; Sugawara T
• Description:
  It has been postulated that an inflammatory response following cutaneous wounding is a prerequisite for healing, and inflammatory cytokines, such as interleukin 6 (lL-6), might intimately involved in this process. Herein, we demonstrate that IL6 deficient transgenic mice (lL-6 KO) displayed significantly delayed cutaneous wound healing compared to wild type control animals, requiring up to three fold longer to heal. This was characterized minimal epithelial bridge formation, decreased inflammation, and granulation tissue formation. Delayed wound healing in IL-6 KO mice was reversed with a single dose of recombinant murine IL6 or intradermal injection of an expression plasmid containing the full length murine IL6 cDNA. We also demonstrate that dexamethasone significantly delays wound healing characterized by delayed re-epithelialization, granulation tissue formation and wound closure. Healing in the dexamethasone treated mice also can be augmented by administration of rmIL-6. In situ hybridization of wound tissue from wild type mice revealed IL-6 mRNA expression primarily in the epidermis at the leading edge of the wound. To further delineate the role of IL-6 in epidermal regeneration, we demonstrate that IL-6 mRNA is expressed and immunoreactive IL-6 is released from normal human epidermal keratinocytes (NHEKs) following in vitro wounding. We also show that IL-1 receptor antagonist will block wound induced IL-6 induction, and that constitutive keratinocyte-derived IL-1 is a major stimulus for IL-6 production in wounded epidermis. These results indicate the importance of IL-6 in wound healing, and a possible therapeutic application of this cytokine. [Description provided by NIOSH]
• Subjects:
  Animals Immune System Laboratories Occupational Health Safety
• Keywords:
  Animal-studies Immune-reaction Immune-system Immunological-tests Injuries Laboratory-animals
• ISSN:
  0892-6638
• Publisher:
  Federation of American Societies for Experimental Biology
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Proceedings
• Place as Subject:
  Oklahoma ; OSHA Region 3 ; OSHA Region 6 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  15
• Issue:
  4
• NIOSHTIC Number:
  nn:20032184
• Citation:
  FASEB J 2001 Mar; 15(4):A355
• Contact Point Address:
  Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Health Effects Laboratory Division, 1095 Willowdale Road, Morgantown, WV 26505-2845
• Editor(s):
  Whelan WJ
• Federal Fiscal Year:
  2001
• Peer Reviewed:
  False
• Source Full Name:
  The FASEB Journal. Experimental Biology 2001, Orlando, Florida, March 13 - April 4, 2001
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:22edb233334b9d3788e6fc566c27d27e61a549f1d5a6ff30e30ae1c37435bf9170dc0afe666117937146c8133a5196df62bdc8d4e862a583c42b8e9cc5db5bea
• Download URL:
  https://stacks.cdc.gov/view/cdc/186226/cdc_186226_DS1.pdf
• File Type:
  [PDF - 305.70 KB ]