Grid-Immunoplotting: A Fast and Simple Technique to Test Multiple Allergens with Small Amounts of Antibody for Cross-Reactivity

Details

• Personal Author:
  Ayuso R ; Lehrer SB ; Reese G ; Schmechel D
• Description:
  Grid-immunoblotting is a procedure that allows the simultaneous testing of up to 20 different antibodies such as monoclonal antibody-containing hybridoma supernatants or human sera for specific antibodies to up to 20 different antigens or allergens on a single sheet of nitrocellulose membrane. Since only 150 to 200 mul of antibody-containing solution are required this technique is uniquely suited to test growing hybridomas and small amounts of sera (e.g. mouse and children's sera). Compared to a standard ELISA, approximately ten times less antibody is needed to obtain the same information. [Description provided by NIOSH]
• Subjects:
  Biological Products Blood Hematologic Tests Hypersensitivity Immune System Occupational Health Safety
• Keywords:
  Allergic-reactions Allergies Biological-agents Blood-analysis Blood-sampling Blood-serum Blood-tests Immunological-tests Immunology
• ISSN:
  0378-4347
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Louisiana ; OSHA Region 3 ; OSHA Region 6 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  151-156
• Volume:
  756
• Issue:
  1
• NIOSHTIC Number:
  nn:20032156
• Citation:
  J Chromatogr B 2001 May; 756(1-2):151-156
• Email:
  greese@tulane.edu
• Federal Fiscal Year:
  2001
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Chromatography B: Biomedical Applications
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:519b262b25d0ea5e8330107876ed1b0b145ae224fafbc7cbb4bec3a051e2cb2adeb71d67c303799086ae66d9bf2d05d49e7adeaf64a698e50019e4fc50c69f2f
• Download URL:
  https://stacks.cdc.gov/view/cdc/186201/cdc_186201_DS1.pdf
• File Type:
  [PDF - 374.05 KB ]