Urinary Benzo[a]pyrene and Its Metabolites as Molecular Biomarkers of Asphalt Fume Exposure Characterized by Microflow LC Coupled to Hybrid Quadrupole Time-of-Flight Mass Spectrometry

Details

• Personal Author:
  Afshari A ; Frazer DG ; Goldsmith T ; Law B ; Lewis, Daniel M. ; Moseley A ; Simpson J ; Stone S ; Wang JJ
• Description:
  As a step to study the health effects of asphalt fume exposure, an analytical method was developed to characterize benzo[a]pyrene and its hydroxy metabolites in the urine of asphalt fume-exposed rats. This method is based on microflow liquid chromatography (LC) coupled to hybrid quadrupole orthogonal acceleration time-of-flight mass spectrometry (Q-TOFMS). Twenty-four female Sprague-Dawley rats were used in the experiment, with 8 as controls and 16 exposed to asphalt fumes in a whole-body inhalation chamber for 10 days (4 h/day). Generated at 150 C, the asphalt fume concentration in the animal exposure chamber ranged 76-117 mg/m3. In the urine of the asphalt fume-exposed rats, benzo[a]pyrene and its metabolites of 3-hydroxybenzo[a]pyrene, benzo[a]pyrene-7,8-dihydrodiol(+/-), and benzo[a]pyrene-7,8,9,10-tetrahydrotetrol(+/-) were identified, and their concentrations were determined at 2.19 +/- 0.49, 16.17 +/- 0.3, 6.28 +/- 0.36, and 29.35 +/- 0.26 ng/100 mL, respectively. The metabolite concentrations from the controlled group, however, were either under the detection limits or at a relatively very low level (0.19 +/- 0.41 ng/100 mL for benzo[a]pyrene-7,8,9,10-tetrahydrotetrol metabolite). The results clearly indicate that the benzo[a]pyrene and its hydroxy metabolites were significantly elevated (p < 0.001) in the urine of asphalt fume-exposed rats relative to controls. The study also demonstrated that the combination of microflow LC separation and collision-induced dissociation leading to a characteristic fragmentation pattern by hybrid Q-TOFMS offers a distinct advantage for the identifications and characterizations of the benzo[a]pyrene metabolites. [Description provided by NIOSH]
• Subjects:
  Animals Benzopyrenes Chromatography Energy Metabolism Environmental Monitoring Hydrocarbons Laboratories Mass Spectrometry Occupational Health Safety
• Keywords:
  Analytical-methods Animal-studies Asphalt-fumes Chromatographic-analysis Exposure-assessment Hydroxy-compounds Laboratory-animals Mass-spectrometry Metabolites
• ISSN:
  0003-2700
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  75
• Issue:
  21
• NIOSHTIC Number:
  nn:20032147
• Citation:
  Anal Chem 2003 Nov; 75(21):5953-5960
• Contact Point Address:
  Jin J. Wang, National Institute for Occupational Safety and Health, Health Effects laboratory Division, 1095 Willowdale Road, Morgantown, WV 26505
• Email:
  juw9@cdc.gov
• CAS Registry Number:
  Benzo[a]pyrene (CAS RN 50-32-8) ; Benzo[a]pyrene-7,8-dihydrodiol (CAS RN 13345-25-0)
• Federal Fiscal Year:
  2004
• Peer Reviewed:
  True
• Source Full Name:
  Analytical Chemistry
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:4c7a7132eba83c9acae69df21a8f0238a617713ed6d59ed9106f95a45fde7af03f125c7d6b4e693798017b73cdda366f487158b1de6da995d211aaaafcf900b8
• Download URL:
  https://stacks.cdc.gov/view/cdc/186194/cdc_186194_DS1.pdf
• File Type:
  [PDF - 123.48 KB ]