Proteomic Analysis of Bronchoalveolar Lavage Fluid: Effect of Acute Exposure to Diesel Exhaust Particles in Rats

Details

• Personal Author:
  Castranova, Vincent ; Dennis WE ; Knechtges PL ; Lewis JA ; Rao KMK ; Vallyathan V ; Castranova, Vincent ; Dennis WE ; Knechtges PL ; Lewis JA ; Rao KMK ; Vallyathan V
• Description:
  BACKGROUND: Inhalation of diesel exhaust particles (DEPs) is characterized by lung injury and inflammation, with significant increases in the numbers of polymorphonuclear leukocytes and alveolar macrophages. This influx of cellular infiltrates is associated with the activation of multiple genes, including cytokines and chemokines, and the production of reactive oxygen species. OBJECTIVE: The pathogenesis of the lung injury is not fully understood, but alterations in the presence or abundance of a number of Proteins in the lung have been observed. Our objective in this study was to further characterize these changes and to ask whether additional changes could be discerned using modem proteomic techniques. METHODS: The present study investigate; global alterations in the proteome of bronchoalveolar lavage fluid taken from rats 1, 7, or 30 days after exposure to 5, 3 5, or 50 mg/kg of animal weight of DEPs. RESULTS: Analysis by surface-enhanced laser desorption/ionization-time of flight mass spectrometry identified two distinct peaks that appeared as an acute response postexposure at all doses in all animals. We identified these two peaks, with mass to charge ratios (m/z) of 9,100 and 10,100, as anaphylatoxin C3a and calgranulin A by additional mass spectral investigation using liquid chromatography coupled to mass spectrometry. CONCLUSIONS: With this approach, we found a number of inflammatory response proteins that may be associated with the early phases of inflammation in response to DEP exposure. Further studies are warranted to determine whether serum levels of these proteins could be markers of diesel exhaust exposure in workers. [Description provided by NIOSH]
• Subjects:
  Animals Asthma Asthma, Occupational Lung Lung Diseases Occupational Health Particulate Matter Respiratory System Respiratory Tract Diseases Safety Vehicle Emissions

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• Keywords:
  Animal-studies Bronchial-asthma Cellular-reactions Cellular-respiration Diesel-emissions Diesel-exhausts Inhalation-studies Lung-cells Lung-disorders Lung-function Particle-aerodynamics Particulates Pulmonary-disorders Pulmonary-system-disorders Respiratory-system-disorders

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• ISSN:
  0091-6765
• Document Type:
  Text
• Genre:
  Journal Article ; Journal Article
• Place as Subject:
  Maryland ; OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH) ; National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division ; HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health ; Workplace Safety & Health
• Location:
  North America ; North America
• Volume:
  115
• Issue:
  5
• NIOSHTIC Number:
  nn:20032113
• Citation:
  Environ Health Perspect 2007 May; 115(5):756-763
• Contact Point Address:
  JA Lewis, U.S. Army Center for Environmental Health Research, 568 Doughten Dr., Ft. Detrick, MD 21740
• Email:
  john.a.lewis1@us.army.mil
• Federal Fiscal Year:
  2007
• NORA Priority Area:
  Manufacturing ; Manufacturing
• Peer Reviewed:
  True
• Source Full Name:
  Environmental Health Perspectives
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:bdd7596788da297851bf17679bc6c6451e1a3a957060b8a399cac5c47afdd907842759a3c1c828fb9b38ecd2d189bad59f468756cd2edc8c589dcd3fbc3ee86b
• Download URL:
  https://stacks.cdc.gov/view/cdc/186165/cdc_186165_DS1.pdf
• File Type:
  [PDF - 599.81 KB ]