Field Evaluation of a Novel Personal Cyclone Sampler for Collection of Fungal Spores

Details

• Personal Author:
  Chen TH ; Macher JM ; Rao, Carol Y.
• Description:
  Chen et a1. (2004) described a personal aerosol sampler based on cyclone principles that uses a 1.5-ml microcentrifuge tube for particle collection. These authors evaluated the collection efficiency for aerosol particles in the size range of fungal spores for different types of microcentrifuge tubes with and without a polyethylene glycol coating or water. They also compared sampler performance at two airflow rates (2 and 4 L min-1). The microcentrifuge-tube sampler was determined to have an aspiration efficiency of 100% in calm air for particles up to 16 microm. At 4 L min-1, the sampler collected nearly 100% of >3 microm particles and >90% ofpartic1es between 2.5 and 3 microm with a 50% cutoff diameter d(50) of 1.5 microm. The current evaluation includes (i) long-term field trials (collection time: 7-19 hours) at an outdoor location with abundant naturally generated airborne fungal spores during a period of peak release (March-May); and (ii) short- and long-term chamber tests (collection time: 10-40 min and 1.3-5.3 hours, respectively) in a sealed glove box with two types of laboratory-generated airborne fungal spores (Aspergillus versicolor and Scopulariopsis brevicaulis). Only preliminary results of the fie1d evaluation are reported here. The overall goal of the project was to evaluate the collection of a personal, cyclone, air sampler relative to widely used environmental samplers (i.e., a filter sampler, another cyclone samp1er, and two types of s1it impactors) for ana1ysis by advanced techniques, such as polymerase chain reaction (PCR) and immunological assays. [Description provided by NIOSH]
• Subjects:
  Aerosols Fungi Microbiology Occupational Health Safety
• Keywords:
  Aerosol-sampling Microorganisms Samplers Sampling-equipment
• ISBN:
  9780978873509
• Publisher:
  American Association for Aerosol Research
• Document Type:
  Text
• Funding:
  Contract
• Genre:
  Abstract or Summary
• Place as Subject:
  California ; Georgia ; Minnesota ; OSHA Region 3 ; OSHA Region 4 ; OSHA Region 5 ; OSHA Region 9 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• NIOSHTIC Number:
  nn:20031880
• Citation:
  Proceedings of the Seventh International Aerosol Conference, September 10-15, 2006, St. Paul, Minnesota. Biswas P, Chen DR, Hering S, eds. Mount Laurel, NJ: American Association for Aerosol Research, 2006 Sep; :512-513
• Editor(s):
  Biswas P; Chen DR; Hering S
• Federal Fiscal Year:
  2006
• Peer Reviewed:
  False
• Source Full Name:
  Proceedings of the Seventh International Aerosol Conference, September 10-15, 2006, St. Paul, Minnesota
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:b70b0dcbe82eb4bef0193b7dfd5afea6dac43aafe7674efb778705bffd639347d7c2986eaa6bf7bef8366eb01fc785809f36576282a0d3ce4269017da3f03b39
• Download URL:
  https://stacks.cdc.gov/view/cdc/185996/cdc_185996_DS1.pdf
• File Type:
  [PDF - 2.27 MB ]