Development of an ELISA Using Polypeptide Fragments of Hemoglobin-Acrylamide Adducts for Biological Monitoring of Acrylamide Exposure

Details

• Personal Author:
  Biagini, Raymond E. ; Snawder, John E. ; Striley CAF
• Description:
  Acrylamide (AA), a probable human carcinogen and genotoxicant, is widely used in industry and agriculture. Acrylamide and its metabolite, glycidamide (GA), are known to form hemoglobin (Hb-AA) adducts that can be used as a biomarkers of exposure. Presently, a modified Edman Degradation followed by GC/MS (ED-GC/MS) analysis is used to measure Hb-AAs. Screening large populations for AA exposure with ED-GC/MS is time consuming, labor intensive and expensive. We are developing an Enzyme Linked Immunosorbant Assay (ELISA) technique to evaluate HIJ.AA with a minimum sample preparation and analysis time. Both AA and GA bind to Hb at the N-terminal valine (N-Term-Val) residue. We synthesized AA- and GA-modified-N-Term-Val decapeptides, homologous with both the alpha and beta chain of Hb. Polyclonal anti-AA- and GA-modlified. N-Term-Val decapeptide antibodies are then used in the development of a high throughput competetive direct ELISA to evaluate AA exposure in humans. [Description provided by NIOSH]
• Subjects:
  Agriculture Biomarkers Energy Metabolism Environmental Monitoring Hematologic Tests Manufacturing And Industrial Facilities Neoplasms Occupational Health Safety
• Keywords:
  Agricultural-chemicals Agricultural-industry Blood-analysis Cancer Exposure-assessment Exposure-levels Industrial-hazards Metabolic-activation Metabolites
• ISSN:
  0065-7727
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DART - Division of Applied Research and Technology
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  226
• NIOSHTIC Number:
  nn:20031842
• Citation:
  Abstr Pap - Am Chem Soc 2003 Sep; 226(Pt 1):U88 033-ARGO
• Contact Point Address:
  Cynthia A. F. Striley, Biomonitoring and Health Assessment Branch, CDC/NIOSH, 4676 Columbia Parkway, Mailstop C-26, Cincinnati, OH 45226
• Email:
  chs3@cdc.gov
• CAS Registry Number:
  Acrylamide (CAS RN 79-06-1)
• Federal Fiscal Year:
  2003
• Peer Reviewed:
  False
• Part Number:
  1
• Source Full Name:
  Abstracts of Papers - American Chemical Society
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:e9e1e496f4defd86620f0f057387237a45ca9ad1a5df2ffb1165e99c29e7d8077bc75dd26e6c4670eb3ff41c101c67c5c424d20adc614c4b8ac30824a5b227eb
• Download URL:
  https://stacks.cdc.gov/view/cdc/185979/cdc_185979_DS1.pdf
• File Type:
  [PDF - 80.94 KB ]