Role of Cell/Cell Interactions in Silica-Induced Production of Inflammatory Mediators in the Lung

Details

• Personal Author:
  Meighan T ; Rao KMK
• Description:
  Exposure of rat alveolar macrophages (AM) to silica in vitro upregulates mRNA expression of a limited number inflammatory mediators compared mRNA upregulation in AM isolated after in vivo exposure to silica (Rao et al., FASEB J. 17:C294, 2003). This observation suggested that cell/cell interactions may play a role in upregulating mRNA expression of some inflammatory mediators. In order to study cell/cell interactions, we isolated AM, rat alveolar type II cells (type II) and lung fibroblasts (LF). mRNA expression of five genes (IL-1(, IL-6, IL-10, iNOS and GM-CSF) was measured by real-time RT/PCR following co-culture of AM with previously cultured type II cells or LF, with or without 200 (g/ml of silica (Min-U-Sil 5). Silica had no additional effects on the expression of any of the cytokines. Therefore, only the data with co-cultures alone, without silica exposure, are shown below: Type II LF AM* Type II* + AM* LF* +AM* IL-1( 1.21 ( 0.07 0.03 ( 0.01 1.1 ( 0.06 N.D. 2.2 ( 1 IL-6 1.27 ( 0.15 0.06 ( 0.26 3.9 ( 3.16 36 ( 26 1730 ( 531 IL-10 1.21 ( 0.11 1.8 ( 0.5 1.4 ( 1.46 0.2 ( 0.3 27 ( 9 iNOS 1.36 ( 0.11 0.15 ( 0.02 1.7 ( 1.63 0.7 ( 0.4 11.5 ( 4 GM-CSF 1.33 ( 0.18 0.06 ( 0.18 3.5 ( 0.71 23 ( 11 40 ( 17 *The numbers in these columns represent mRNA expression (mean ( SEM of at least 3 separate experiments) relative to AM alone (column 2). N.D. not detectable. These data indicate that LF are an important source of inflammatory mediators and that AM/LF interactions play a role in the expression of IL-6, IL-10 and iNOS in basal co-cultures. [Description provided by NIOSH]
• Subjects:
  Cells, Cultured Dust Lung Occupational Health Respiratory Function Tests Respiratory System Respiratory Tract Diseases Safety Silicon Dioxide
• Keywords:
  Cell-alteration Cell-biology Cell-culture-techniques Cell-function Cell-metabolism Cellular-function Cellular-transport-mechanism Dust-analysis In-vitro-studies In-vitro-study Lung-cells Pulmonary-function Pulmonary-function-tests Pulmonary-system-disorders Silica-dusts

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• ISSN:
  0892-6638
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Proceedings
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  18
• Issue:
  5
• NIOSHTIC Number:
  nn:20031575
• Citation:
  FASEB J 2004 Mar; 18(5)(Suppl):A1139-A1140
• Contact Point Address:
  NIOSH, PPRB, HELD, Morgantown, WV 26505
• Federal Fiscal Year:
  2004
• Peer Reviewed:
  False
• Source Full Name:
  The FASEB Journal. Experimental Biology 2004, Washington, DC, April 17-21, 2004
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:65c0f5d023e62d3dc2f5c8cb14e5db227e920ba02c0e65005f9877f3e677287cb6b98d50f4656001061c9f9d9166b863d870fc729cd0133d18c611aac868588e
• Download URL:
  https://stacks.cdc.gov/view/cdc/185824/cdc_185824_DS1.pdf
• File Type:
  [PDF - 204.67 KB ]