Efficacy of Culture Filtrate Protein Preparations from Indian Isolates of M-Tuberculosis to Activate T Cells Derived from Healthy Donors

Details

• Personal Author:
  Orme IM ; Saxena RK ; Siddiqui SM
• Description:
  SETTING: While culture filtrate proteins (CFPs) of Mycobacterium tuberculosis appear to be good vaccine candidates for tuberculosis, only CFPs derived from certain popular laboratory strains of M. tuberculosis have been studied for this purpose. OBJECTIVE: To compare the relative efficacies of CFP preparations from two laboratory strains and four contemporary clinical isolates of M. tuberculosis to induce T-cell activation. DESIGN: CFPs were isolated From six strains of M. tuberculosis and were used to induce 1) T-cell proliferation, 2) IFN-gamma secretion, and 3) IL-12 secretion from peripheral blood derived mononuclear cell (PBMC) preparations from 33 healthy donors. RESULTS: Significant amounts of IL-12 were spontaneously secreted by PBMC preparations; CFP preparations from two clinical isolates (JNU-7 and JNU-51) significantly boosted this response. All six CFP preparations induced IFN-gamma secretion by PBMCs. but those from two contemporary strains of M. tuberculosis (JNU-7 and JNU-22) were most effective in this regard. The effect of CFPs from JNU-7 and JNU-22 was significantly better than those from the laboratory strains (H37Ra and Erdman). Similar results were obtained with the T-cell proliferation parameter. [Description provided by NIOSH]
• Subjects:
  Chemistry Chemistry, Analytic Clinical Laboratory Techniques Laboratories Lung Lung Diseases Occupational Health Respiratory Function Tests Respiratory System Respiratory Tract Diseases Safety

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• Keywords:
  Analytical-chemistry Analytical-methods Analytical-processes Cell-biology Cell-damage Cell-function Cell-metabolism Cell-transformation Cellular-respiration Cellular-transport-mechanism Clinical-chemistry Pulmonary-disorders Pulmonary-function-tests Pulmonary-system-disorders Respiratory-function-tests Respiratory-infections Respiratory-system-disorders

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• ISSN:
  1027-3719
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Colorado ; OSHA Region 3 ; OSHA Region 8 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  4
• Issue:
  10
• NIOSHTIC Number:
  nn:20032343
• Citation:
  Int J Tuberc Lung Dis 2000 Oct; 4(10):980-987
• Contact Point Address:
  Prof R K Saxena, HELD, National Institute of Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505
• Email:
  rajivksaxena@hotmail.com
• Federal Fiscal Year:
  2001
• Peer Reviewed:
  True
• Source Full Name:
  The International Journal of Tuberculosis and Lung Disease
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:3712462bcdddd99ea13539b1dc44bd5b73dcadafdece1ec3c3a03654355d29ab4c3c880d4fcea5fb19207c49f40c6d7cec4a6bcffe5656ad3a6f4c5d1c04be21
• Download URL:
  https://stacks.cdc.gov/view/cdc/186361/cdc_186361_DS1.pdf
• File Type:
  [PDF - 986.44 KB ]