Transmembrane potential changes during phagocytosis in rat alveolar macrophages.

Details

• Personal Author:
  Bowman L ; Castranova, Vincent ; Miles PR
• Description:
  Phagocytosis induced changes in transmembrane potential were studied in rat alveolar macrophages. Alveolar macrophages harvested from male Long-Evans-rats were incubated with 4 milligrams (mg) zymosan (9010724) or 50 micrograms (microg) phorbol-12-myristate-13-acetate (62384) (PMA) and the effects on superoxide-anion (O2-) release and transmembrane potential were monitored for up to 100 minutes. Alveolar macrophages were incubated with 0 to 4mg zymosan or 0 to 16microg/milliliter PMA to assess the concentration effects on O2- release and transmembrane potential. Alveolar macrophages were preincubated with 0, 5, or 150 millimolar (mM) potassium-chloride to depolarize them. They were then incubated with 0 or 4mg zymosan. The effects on O2- release and transmembrane potential were examined. Phagocytosis of either zymosan or PMA by rat alveolar macrophages resulted in membrane depolarization and release of O2-. Membrane depolarization preceded the release of O2-. The magnitude of depolarization and O2- release increased with increasing PMA or zymosan concentration. Preincubation with potassium-ion (K+) induced release of O2-. The magnitude of depolarization induced by 150mM K+ was only about 20 percent of that induced by zymosan. The amount of O2- released was reduced by pretreatment with K+. The authors conclude that release of O2- from rat alveolar macrophages after ingesting foreign particles is associated with depolarization of the cell membrane. [Description provided by NIOSH]
• Subjects:
  Lung Occupational Health Particulate Matter Respiratory System Safety
• Keywords:
  Alveolar-cells Bioelectric-effects Dose-response In-vitro-studies NIOSH-Author Oxidative-processes Particulates Phagocytic-activity Physiological-chemistry
• ISSN:
  0021-9541
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  ALOSH - Appalachian Laboratory for Occupational Safety and Health
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  109-117
• Volume:
  106
• Issue:
  1
• NIOSHTIC Number:
  nn:00184748
• Citation:
  J Cell Physiol 1981 Jan; 106(1):109-117
• Contact Point Address:
  P. R. Miles, Appalachian Laboratory for Occupational Safety and Health, Morgantown, West Virginia 26505
• CAS Registry Number:
  Phenylmercuric acetate (CAS RN 62-38-4) ; Zymosans (CAS RN 9010-72-4)
• Federal Fiscal Year:
  1981
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Cellular Physiology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:b503a00c164505b7fca5514c095e0a6be94b77beaaa9b8895089d3ba1aa6061c839793be3c151007fa5179331d41aff33d2a282201647af48dbf5443d698f087
• Download URL:
  https://stacks.cdc.gov/view/cdc/185691/cdc_185691_DS1.pdf
• File Type:
  [PDF - 608.91 KB ]