Respiratory burst activity in rat alveolar macrophages: particle-stimulated oxygen consumption independent of superoxide anion release.

Details

• Personal Author:
  Castranova, Vincent ; Miles PR ; Van Scott MR
• Description:
  Respiratory burst activity during stimulation of alveolar macrophages was studied in-vitro. The purpose of the study was to test the assumption that oxygen consumption stimulated by exposing alveolar macrophages to xenobiotics was used in releasing superoxide- anion (O2-). Suspensions of alveolar macrophages obtained from male Sprague-Dawley-rats were stimulated with 0 to 3000 micrograms per milliliter (microg/ml) concanavalin-A (11028710) (conA) or 0 to 6.0mg/ml zymosan (9010724) particles. Oxygen consumption and release of hydrogen-peroxide and O2- by the suspensions was monitored. The time course and dose response curves for zymosan and conA stimulated oxygen consumption did not match those for hydrogen- peroxide and O2- production. The maximum rate of oxygen consumption induced by zymosan occurred at a lower concentration than was required to release O2- or hydrogen-peroxide. ConA at concentrations above 600microg/ml decreased oxygen consumption and hydrogen-peroxide release. O2- release increased over the entire range of conA concentrations. Additionally rat alveolar macrophages were preincubated with 0 or 0.12 millimolar cytochrome-c (9007436), followed by 0.1 to 2.0mg/ml zymosan. The effects on oxygen consumption were monitored. Rat alveolar macrophages were treated with 0 or 0.1mg/ml zymosan and 0 or 5microg/ml cytochalasin-B (14930962). The effects on oxygen consumption and O2- and hydrogen- peroxide formation were monitored. Cytochrome-c inhibited zymosan induced oxygen consumption by 40 percent. Cytochalasin-B stimulated zymosan induced oxygen consumption and hydrogen-peroxide release by 98.5 and 44 percent, respectively, but had no effect on O2- production. The authors conclude that all of the stimulated increase in oxygen consumption in alveolar macrophages is not used in generating O2-. Some of the increase may be consumed by mechanical processes involving microfilament activation. [Description provided by NIOSH]
• Subjects:
  Lung Occupational Health Respiratory System Safety
• Keywords:
  In-vitro-studies; Alveolar-cells; Dose-response; Physiological-chemistry; Oxidative-processes; Phagocytic-activity; Oxygen-uptake
• ISBN:
  9780849343322
• Publisher:
  CRC Press
• Document Type:
  Text
• Genre:
  Book
• Place as Subject:
  Florida ; OSHA Region 3 ; OSHA Region 4 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  21-37
• NIOSHTIC Number:
  nn:00184745
• Citation:
  Cellular chemiluminescence. Van Dyke K, Castranova V, eds. Boca Raton, FL: CRC Press, 1987 Jul; :21-37
• CAS Registry Number:
  Concanavalin A (CAS RN 11028-71-0) ; Cytochalasin B (CAS RN 14930-96-2) ; Cytochrome c (CAS RN 9007-43-6) ; Zymosans (CAS RN 9010-72-4)
• Editor(s):
  Van Dyke K; Castranova V
• Federal Fiscal Year:
  1987
• Peer Reviewed:
  False
• Source Full Name:
  Cellular chemiluminescence
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7dc847c09aedf140bc731323e8446b4703641ae07d6ad9e7b7238120dab30b8f7dcaa9b938d34d035f87873a0fe7fec262d5d13dcaf290925445e82b6de3b57d
• Download URL:
  https://stacks.cdc.gov/view/cdc/242697/cdc_242697_DS1.pdf
• File Type:
  [PDF - 168.02 KB ]