Variation between three strains of rat: inhibition of neurotoxic esterase and acetylcholinesterase by tri-o-cresyl phosphate.

Details

• Personal Author:
  Abou-Donia M ; Carrington CD
• Description:
  The effect of the strain of rodent on the results of a test for neurotoxicity was studied in rats exposed to the organophosphorus compound tri-o-cresyl-phosphate (78308) (TOCP). Sprague-Dawley-rats (SD), Long-Evans-rats (LE), and Fischer-344-rats (F344) were administered TOCP by oral gavage at doses on the basis of their established delayed neurotoxicity in LE (290, 580, or 1160mg/kg); animals were sacrificed 48 hours after dosing and the brain homogenized for measurement of inhibition of neurotoxic-esterase (NTE) and acetylcholinesterase (AChE) as end points for organophosphorus compound induced delayed neurotoxicity and acute toxicity, respectively. Differences in basal levels among the strains were found for NTE (LE greater than F344 greater than SD) but not AChE. Strain differences in inhibition by TOCP were found with both assays, with SD rats much less sensitive to esterase inhibition than LE or F344-rats; the median effective dose values for NTE inhibition were estimated to be 458, 209, and 288mg/kg for SD, F344, and LE rats, respectively. Liver microsomes from the F344 rats had less cytochrome-P-450 than those from the other two strains. The authors conclude that differences in the ability of the strains to form or inactivate the active metabolite of TOCP may account for the observed variations in this study; the results may be important in selecting a strain for the study of the toxic effects of organophosphorus compounds in rats. [Description provided by NIOSH]
• Subjects:
  Enzymes Hazardous Substances Laboratories Nervous System Occupational Health Organophosphates Safety Toxicology
• Keywords:
  Comparative-toxicology Enzyme-activity Laboratory-animals Neurotoxicology NIOSH-Grant NIOSH-Publication Organo-phosphorus-compounds Toxic-dose Toxic-effects
• ISSN:
  0098-4108
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  North Carolina ; OSHA Region 4
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  259-268
• Volume:
  25
• Issue:
  3
• NIOSHTIC Number:
  nn:00184482
• Citation:
  J Toxicol Environ Health 1988 Jan; 25(3):259-268
• Contact Point Address:
  Pharmacology Duke University Department of Pharmacology Durham, N C 27710
• CAS Registry Number:
  o-Cresyl phosphate (CAS RN 78-30-8)
• Federal Fiscal Year:
  1988
• Performing Organization:
  Duke University, Durham, North Carolina
• Peer Reviewed:
  True
• Start Date:
  19790401
• Source Full Name:
  Journal of Toxicology and Environmental Health
• End Date:
  19980331
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:9047a270c48430fd4876c823a529546d8df4f5dee72ceeb25233e6db8b14879981c46c2a519884a8c8088ddf559e729da28e2efad5a4eb0b333f7bde134df092
• Download URL:
  https://stacks.cdc.gov/view/cdc/185680/cdc_185680_DS1.pdf
• File Type:
  [PDF - 730.96 KB ]