Chloroform induction of ornithine decarboxylase antizyme (ODC-AZ) in male rat liver.

Details

• Personal Author:
  DeAngelo AB ; Pereira MA ; Savage RE Jr.
• Description:
  The induction of ornithine-decarboxylase antizyme by chloroform (67663) stimulation was assessed in male and female Charles River CDF Fischer-344-rats. The animals were given chloroform at a dose of 3 millimoles per kilogram body weight by daily intraperitoneal injection for a period of 1, 3, or 7 days. Ornithine-decarboxylase antizyme activity was determined following the exposure period by inhibition of ornithine-decarboxylase activity in an hepatic microsomal preparation. Chloroform stimulation of ornithine- decarboxylase activity was greatest at 18 hours after a single treatment, and repeated dosing progressively reduced the response of the liver microsomal enzyme. DEAE-cellulose chromatography showed increased ornithine-decarboxylase antizyme activity in liver microsomal fractions from treated male animals only. The antizyme activity was recoverable from the chloroform treated male rats and positive treated controls by Sephadex-G75 molecular sieving. The authors conclude that the inhibition of microsomal ornithine- decarboxylase after repeated chloroform stimulation is due in part to ornithine-decarboxylase antizyme activity in male, but not in female, rats. [Description provided by NIOSH]
• Subjects:
  Carcinogens Enzymes Hydrocarbons Laboratories Liver Occupational Health Safety Sex Characteristics
• Keywords:
  Carcinogenesis Chlorinated-hydrocarbons Enzymatic-effects Enzyme-activity Hepatotoxicity Laboratory-animals Liver-microsomal-enzymes NIOSH-Author Sex-factors
• ISSN:
  0098-4108
• Document Type:
  Text
• Genre:
  Journal Article
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  97-101
• Volume:
  25
• Issue:
  1
• NIOSHTIC Number:
  nn:00182845
• Citation:
  J Toxicol Environ Health 1988 Sep; 25(1):97-101
• CAS Registry Number:
  Chloroform (CAS RN 67-66-3)
• Federal Fiscal Year:
  1988
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Toxicology and Environmental Health
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:8411f567effc96f88f40b9243f90bafe35e160b0b44f920e83de183cfa7be03437524e73b1e4badc1b989f04197484a67d10b7d805d79a077da70d3ebba018cb
• Download URL:
  https://stacks.cdc.gov/view/cdc/185643/cdc_185643_DS1.pdf
• File Type:
  [PDF - 426.65 KB ]