Heterogeneity in dog red blood cells: sodium and potassium transport.

Details

• Personal Author:
  Castranova, Vincent ; Hoffman JF
• Description:
  Heterogeneous characteristics of sodium ion (Na+) and potassium ion (K+) transport mechanisms in dog red blood cells (RBCs) were evaluated. Blood was drawn from adult dogs and the RBCs isolated by centrifugation. After incubation in isotonic potassium-chloride (KCl), the cells were further separated by centrifugation into subgroups with different cell volumes and different transport characteristics; red cells which swelled in isotonic KCl exhibited a higher permeability to radiolabeled K+ and possessed a greater volume dependence for transport of K+ than those red cells which shrank. A high Na+ permeability characterized cells which shrank in isotonic KCl; such cells exhibited a larger volume dependent Na+ flux than those red cells which swelled. The two subgroups of red cells did not appear to represent cell populations of different ages. The authors conclude that the population of normal RBCs in dogs is heterogeneous in that the volume dependent changes in Na+ and K+ permeability are distributed between different cell types rather than representing a single cell type which reciprocally changes its selectivity for the two ions; the basis for the volume response as well as the differential ion selectivity of cells in the two subgroups is not known. [Description provided by NIOSH]
• Subjects:
  Animals Biochemistry Occupational Health Safety
• Keywords:
  Animal-studies Biochemical-analysis Cell-function Kinetics NIOSH-Author Physiological-function Red-blood-cells Transport-mechanisms
• ISSN:
  0022-1295
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Connecticut ; OSHA Region 1 ; OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  ALOSH - Appalachian Laboratory for Occupational Safety and Health
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  61-71
• Volume:
  73
• Issue:
  1
• NIOSHTIC Number:
  nn:00182504
• Citation:
  J Gen Physiol 1979 Jan; 73(1):61-71
• Federal Fiscal Year:
  1979
• Peer Reviewed:
  True
• Source Full Name:
  Journal of General Physiology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:65935bc565f0c3b4ddbeee68fe5d292033d65b144c00d31e050a518cf4d0afd931a1ae122b6c9a93eb6b2c9437993096f28103ce319cc9b6ee6f0952cc06c2a8
• Download URL:
  https://stacks.cdc.gov/view/cdc/185638/cdc_185638_DS1.pdf
• File Type:
  [PDF - 591.40 KB ]