Demonstration of inflammatory cell population changes in rat lungs in response to intratracheal instillation of spring wheat dust using lung enzymatic digestion and centrifugal elutriation.

Details

• Personal Author:
  Keller GE III ; Lewis, Daniel M. ; Olenchock, Stephen A.
• Description:
  The response of inflammatory cells in the lung and peripheral blood was determined during short term time course exposures to inhaled grain dust in specific pathogen free Lewis-rats. Grain dust suspensions were prepared from airborne spring wheat dust collected from port grain terminals in the Superior-Duluth region; dust was suspended in saline at 4 milligrams per millimeter (mg/ml). Spring wheat dust suspension or saline (control) was instilled in rats intratracheally; 6 or 24 hours later, rats were killed. Alveolar macrophages were collected by bronchoalveolar lavage. Significant increases were noted in neutrophils at both the 6 and 24 hour time periods in animals receiving the dust. Lymphocyte numbers were significantly higher in the control group than in the dust treated group. Significantly elevated numbers of granulocytes were present in the dust exposed lung and airways surfaces as determined by bronchoalveolar lavage. On examination of enzymatic digestion of rat lungs a significantly higher number of neutrophils was found in the dust exposed animals at both 6 and 24 hours than was seen for the controls. A large increase in the number of neutrophils in the alveoli was found on histological examination of the dust treated animals at 6 hours. No specific precipitating antibodies were noted on counterimmunoelectrophoresis of random serum samples from control and exposed rats. The data were consistent with a time course for an inflammatory process subsequent to the instillation of spring wheat dust. The authors conclude that intratracheal instillation of airborne grain dust resulted in inflammatory cell activation. The neutrophil was the primary responding cell with increases noted in neutrophils in the peripheral blood, bronchoalveolar spaces, and lung interstitium. [Description provided by NIOSH]
• Subjects:
  Air Pollutants, Occupational Blood Dust Laboratories Lung Occupational Health Particulate Matter Respiratory System Safety
• Keywords:
  Agriculture Airborne-dusts Author Keywords: Inflammation Blood-cells Centrifugal Elutriation Dust-inhalation Enzymatic Digestion Grain-dusts In-vivo-studies Laboratory-animals Lung Lung-cells Neutrophils NIOSH-Author Occupational Health Plant-dusts Rats Spring Wheat Dust

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• ISSN:
  0147-9571
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  219-226
• Volume:
  10
• Issue:
  3
• NIOSHTIC Number:
  nn:00180886
• Citation:
  Comp Immunol Microbiol Infect Dis 1987 Jan; 10(3-4):219-226
• Federal Fiscal Year:
  1987
• Peer Reviewed:
  True
• Source Full Name:
  Comparative Immunology, Microbiology and Infectious Diseases
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:136ca50cc36c67b8625850b0f7d0f8f7e795cf07e919afecd39be8e9d34b4f6afd534251c82bbebab831b2ba228e6364515f16c81abc000263431eead8e39190
• Download URL:
  https://stacks.cdc.gov/view/cdc/185565/cdc_185565_DS1.pdf
• File Type:
  [PDF - 561.27 KB ]