Factors affecting lipid peroxidation in guinea-pig adrenal microsomes.

Details

• Personal Author:
  Brogan WC III ; Colby HD ; Miles PR
• Description:
  The effects of several endogenous substances on the oxidative degradation of unsaturated lipids (lipid peroxidation) in the adrenal and liver in-vitro were compared. Adrenal and hepatic microsomes were isolated from male English-Short-Hair-guinea-pigs, and effects of NADPH, ferrous ion (Fe+2), ferric ion (Fe+3), and L- ascorbate on lipid peroxidation were evaluated. Lipid peroxidation was measured by malonaldehyde (MDA) production, as detected by the thiobarbituric-acid test. Fe+2 caused a concentration dependent increase in lipid peroxidation by adrenal microsomes between 10(-6) and 10(-3) molar (M). Fe+3 was much less effective in adrenals, while these ions had similar activities in liver microsomes. Ascorbate at 10(-4)M interacted synergistically with Fe+2 at low but not high concentrations to increase lipid peroxidation in adrenal microsomes. Ascorbate acted synergistically with Fe+3 at all concentrations. Higher concentrations of ascorbate reduced lipid peroxidation, possibly due to manifestation of its antioxidant properties. These effects were seen in heat treated microsomes as well, indicating the nonenzymatic nature of the inhibited activity. NADPH alone had no effect on adrenal microsomes but showed a dose dependent stimulation of enzymatic peroxidation in the presence of low levels of Fe+2 and, to some extent, Fe+3. At higher iron concentrations, NADPH inhibited nonenzymatic lipid peroxidation. The capacity for nonenzymatic lipid peroxidation was greater in adrenal versus hepatic microsomes. Enzymatic lipid peroxidation was stimulated by NADPH in the absence of Fe+2 for hepatic, but not adrenal microsomes. The authors conclude that multiple interactions between a variety of substances can have profound effects on lipid peroxidation in adrenals, and that liver and adrenal lipid peroxidation processes are controlled differently. [Description provided by NIOSH]
• Subjects:
  Ferrous Compounds Laboratories Lipids Metals, Heavy Occupational Health Safety
• Keywords:
  Ascorbate Author Keywords: Lipid Peroxidation Cell-metabolism Guinea-pig Adrenal Microsome Heavy-metals In-vitro-studies Iron Laboratory-animals Lipid-peroxidation Liver-microsomal-metabolism Malonaldehyde Microsomal-enzymes NADPH NIOSH-Author Vitamins

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• ISSN:
  0006-3002
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  ALOSH - Appalachian Laboratory for Occupational Safety and Health
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  230-238
• Volume:
  663
• Issue:
  1
• NIOSHTIC Number:
  nn:00180781
• Citation:
  Biochim Biophys Acta 1981 Jan; 663(1):230-238
• Contact Point Address:
  Howard D. Colby, Appalachian Laboratory for Occupational Safety and Health, Morgantown, WV 26505 (U.S.A.)
• Federal Fiscal Year:
  1981
• Peer Reviewed:
  True
• Source Full Name:
  Biochimica et Biophysica Acta
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:b1ef259826d905437497b8e5071dc4af2e1febb71576b568e71db0cf8c2354cd17b56663f33f8691aa08e998f2e73dec7ee072daad7a07b23b8d3b1956bc530c
• Download URL:
  https://stacks.cdc.gov/view/cdc/185561/cdc_185561_DS1.pdf
• File Type:
  [PDF - 687.95 KB ]