Lipid peroxidation in guinea pig lung microsomes.

Details

• Personal Author:
  Colby HD ; Miles PR ; Wright JR ; Colby HD ; Miles PR ; Wright JR
• Description:
  Pulmonary microsomal lipid peroxidation was studied in-vitro with regard to the effects of NADPH, ferrous ion (Fe(2+)), ferric ion (Fe(3+)), and ascorbate. Lungs were dissected from male English- short-hair-guinea-pigs, and the microsomes were obtained from lung homogenates by differential centrifugation. Lipid peroxidation was determined by the production of malonaldehyde in the presence or absence of the substances used to initiate or modify peroxidation. The addition of 2.5 millimolar (mM) Fe(2+), Fe(3+), or ascorbate increased pulmonary microsomal lipid peroxidation in the increasing order of Fe(3+), ascorbate, and Fe(2+). The addition of ascorbate enhanced Fe(3+) stimulated lipid peroxidation but reduced the magnitude of the Fe(2+) stimulated lipid peroxidation. NADPH stimulated lipid peroxidation in normal pulmonary microsomes but not in heat treated preparations or preparations containing 1mM ethylenediaminetetraacetic-acid. Fe(2+) and phosphate were required for maximal microsomal stimulation by NADPH, but the addition of NADPH to the Fe(2+) medium decreased microsomal stimulation when the iron concentration was greater than 1mM. The results were compared to similar experiments using liver microsomal preparations, and the authors conclude that there are significant differences in the behavior of pulmonary and hepatic microsomes. [Description provided by NIOSH]
• Subjects:
  Enzymes Laboratories Lipids Lung Occupational Health Respiratory System Safety
• Keywords:
  Author Keywords: Lipid Peroxidation Enzymatic-effects Fe 2+ Fe3+: Ascorbate Guinea Pig Lung Microsome In-vitro-studies Laboratory-animals Lipid-peroxidation Lung-cells Malonaldehyde Production Microsomal-enzymes NIOSH-Author Physiological-response Pulmonary-function Pulmonary-system

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• ISSN:
  0006-3002
• Document Type:
  Text
• Genre:
  Journal Article ; Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH) ; National Institute for Occupational Safety and Health (NIOSH)
• Division:
  ALOSH - Appalachian Laboratory for Occupational Safety and Health ; ALOSH - Appalachian Laboratory for Occupational Safety and Health
• Topic:
  Workplace Safety & Health ; Workplace Safety & Health
• Location:
  North America ; North America
• Pages in Document:
  374-384
• Volume:
  619
• Issue:
  2
• NIOSHTIC Number:
  nn:00176510
• Citation:
  Biochim Biophys Acta 1980 Aug; 619(2):374-384
• Federal Fiscal Year:
  1980
• Peer Reviewed:
  True
• Source Full Name:
  Biochimica et Biophysica Acta
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:3f3f618c4f6f744af45ec1222782f84a8ab56a1e92f0e4ca84a65499f5c567bcf429d0a85420c97e0b438c2117b002e3d12e52804d3f42162932d70c7fe09c51
• Download URL:
  https://stacks.cdc.gov/view/cdc/185431/cdc_185431_DS1.pdf
• File Type:
  [PDF - 901.82 KB ]