In vitro inactivation of plasma alpha1-proteinase inhibitor by epoxides and 1,2-dihaloethanes.

Details

• Personal Author:
  Ansari GA ; Barton BK ; Gan JC ; Ansari GA ; Barton BK ; Gan JC
• Description:
  The inactivation of alpha1-proteinase inhibitor (a1PI) by epoxides and 1,2-dihaloethanes was studied in-vitro. a1PI and whole plasma were incubated with styrene-oxide (96093), ethylene-oxide (75218), propylene-oxide (75569), 1,2-dichloroethane (107062) (DECE), or 1,2- dibromoethane (106934) (DBE) for 2 hours. The effects on elastase inhibiting capacity (EIC) and trypsin inhibiting capacity (TIC) were examined. The extent of alkylation of amino groups on a1PI by the compounds was determined. The epoxides reduced the EIC and TIC of a1PI and plasma. Styrene-oxide was the most effective inhibitor, followed by ethylene-oxide and propylene-oxide in that order. Styrene-oxide modified four amino groups on a1PI. DCE and DBE reduced the EIC and TIC of a1PI, but to a lesser extent than that produced by the epoxides. DCE was more effective than DBE. The concentrations required for inhibiting EIC or TIC of whole plasma were generally higher than those required for inactivating a1PI. DECE alkylated three amino groups on a1PI and DBE two amino groups. The authors conclude that a1PI in whole plasma is inhibited at higher concentrations than pure a1PI. It is considered unlikely that humans will be exposed to such high concentrations except in the case of accidental acute exposure. Since in-vitro studies do not address the effect of metabolites of the chemicals on the inhibitory activity of a1PI, in-vivo studies are needed. [Description provided by NIOSH]
• Subjects:
  Blood Bromine Compounds Chlorine Compounds Epoxy Resins Occupational Health Safety
• Keywords:
  Blood-plasma Dose-response Epoxy-compounds In-vitro-studies NIOSH-Grant NIOSH-Publication Organo-bromine-compounds Organo-chlorine-compounds Physiological-chemistry
• ISSN:
  0090-4341
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article ; Journal Article
• Place as Subject:
  OSHA Region 6 ; Texas
• CIO:
  National Institute for Occupational Safety and Health (NIOSH) ; National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health ; Workplace Safety & Health
• Location:
  North America ; North America
• Volume:
  17
• Issue:
  4
• NIOSHTIC Number:
  nn:00180348
• Citation:
  Arch Environ Contam Toxicol 1988 Jul; 17(4):537-542
• Contact Point Address:
  Human Biol Chem and Genetics University of Texas Med BR Dept of Human Biol Chem&gene Galveston, Tex 77550-2774
• CAS Registry Number:
  (±)-Propylene oxide (CAS RN 75-56-9) ; 1,2-Dibromoethane (CAS RN 106-93-4) ; 1,2-Dichloroethane (CAS RN 107-06-2) ; Ethylene oxide (CAS RN 75-21-8) ; Styrene oxide (CAS RN 96-09-3) ; (±)-Propylene oxide (CAS RN 75-56-9) ; 1,2-Dibromoethane (CAS RN 106-93-4) ; 1,2-Dichloroethane (CAS RN 107-06-2) ; Ethylene oxide (CAS RN 75-21-8) ; Styrene oxide (CAS RN 96-09-3)
• Federal Fiscal Year:
  1988
• Performing Organization:
  University of Texas Medical Branch, Galveston, Texas
• Peer Reviewed:
  True
• Start Date:
  19860101
• Source Full Name:
  Archives of Environmental Contamination and Toxicology
• End Date:
  19960630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:0fe2b5aff5e898e65cc8a1955d4cb620c30f4824940f208d21fe2ef3a4a9942aa02e16779ef682e72ff8e029ceefe9ee789d9e8da8ae58f1b32597b1ed109e31
• Download URL:
  https://stacks.cdc.gov/view/cdc/185547/cdc_185547_DS1.pdf
• File Type:
  [PDF - 672.54 KB ]