Mutagenicity studies of ambient airborne particles. II. Comparison of extraction methods.

Details

• Personal Author:
  Krishna G ; Nath J ; Ong T ; Whong Z
• Description:
  The mutagenic activities of ambient airborne particles were assessed and compared in the Ames Salmonella microsome assay system and the arabinose resistance Salmonella assay system after acetone extraction by shaking, soxhletion, or sonication. Collection of the airborne particles was done during the summer of 1982 using a high volume sampler with glass microfiber filters at a height of 10 meters above ground. Sampling proceeded continuously over a period of 48 hours at a flow rate of 60 cubic feet per minute. Each extraction method was tested using several different duration times to determine optimum duration time for extraction. The three methods were then compared for extraction efficiency using the optimum duration time. Tester strains used were (TA-98) and (TA- 100) for the Ames Salmonella assay and (SV-50) for the arabinose resistance test. The mutagenic activity of extracted airborne particles was greatest at 4 hours for shaking, 1 hour for soxhlet extraction, and 1/8 of a minute for sonication. The results of the Salmonella assay system and the arabinose resistance test were comparable with regard to extraction time, and similar responses were observed with and without microsomal activation. There were no significant differences in the mutagenic responses of the three strains used. The order of mutagenic activity of extracts from highest to lowest at optimum extraction time was sonication, shaking, and soxhletion. The authors conclude that acetone extraction by sonication is the best method for extraction of mutagens from airborne particles. [Description provided by NIOSH]
• Subjects:
  Air Pollutants, Occupational Laboratories Microbiology Mutagens Occupational Health Particulate Matter Safety Solvents
• Keywords:
  Airborne-particles Gene-mutation Laboratory-techniques Microorganisms Mutagenesis NIOSH-Author Organic-solvents Sample-preparation Solvent-extraction
• ISSN:
  0027-5107
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  121-128
• Volume:
  124
• Issue:
  2
• NIOSHTIC Number:
  nn:00176517
• Citation:
  Mutat Res 1983 Nov; 124(2):121-128
• Federal Fiscal Year:
  1984
• Peer Reviewed:
  True
• Source Full Name:
  Mutation Research
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:a8122ce9d2a349c238f8b243145f6e887482e524142f706459d960157b9c724994a11ed6dee463d9ddea9e094f1f282e4829cbb1650050d867cc710184d87f81
• Download URL:
  https://stacks.cdc.gov/view/cdc/185437/cdc_185437_DS1.pdf
• File Type:
  [PDF - 336.49 KB ]