Catabolism of rat surfactant disaturated phosphatidylcholines during incubation of alveolar lavage materials in vitro at 37 degrees C.

Details

• Personal Author:
  Bowman L ; Castranova, Vincent ; Miles PR
• Description:
  The catabolism of rat surfactant disaturated phosphatidylcholines was investigated during incubation of rat alveolar lavage materials in-vitro at 37 degrees-C. Male Sprague-Dawley-rats were given intravenous injections of either tritiated palmitate or tritiated choline and sacrificed 22 hours post injection. The amounts of the labeled chemicals in the endogenous surfactant disaturated phosphatidylcholines were determined before and at various times after incubation of alveolar lavage material. The time course of disappearance of endogenous disaturated phosphatidylcholines was linear for approximately 10 minutes post incubation, and the time course for the disappearance of exogenous dipalmitoylphosphatidylcholine was linear for approximately 3 minutes. Catabolism of the disaturated compound was 76 percent complete after 4 hours at 37 degrees-C but only 8 percent complete after 4 hours at 2 degrees-C. The products of the catabolism of choline and palmitate labeled disaturated phosphatidylcholines were free palmitate and water soluble choline derivatives. No lysophosphatidylcholines were found. No correlation between the lactate-dehydrogenase level of the lavage fluid and the amount of disaturated phosphatidylcholine catabolism was found. The authors conclude that the phospholipases present in rat lavage fluid are responsible for the catabolism of disaturated phosphatidylcholines and that the occurrence of these enzymes in the lavage fluid is not due to cellular damage. [Description provided by NIOSH]
• Subjects:
  Biological Assay Enzymes Laboratories Lipids Lung Occupational Health Respiratory System Safety
• Keywords:
  Alveolar-cells Analytical-methods Author Keywords: Lung Surfactant Bioassays Cell-metabolism Enzymatic-effects In-vitro-studies Incubation Temperature Laboratory-animals NIOSH-Author Phosphatidylcholine Catabolism Physiological-response Pulmonary-function

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• ISSN:
  0006-3002
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  ALOSH - Appalachian Laboratory for Occupational Safety and Health
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  39-44
• Volume:
  836
• Issue:
  1
• NIOSHTIC Number:
  nn:00174925
• Citation:
  Biochim Biophys Acta 1985 Aug; 836(1):39-44
• Contact Point Address:
  P. R. Miles, Physiology Section, ALOSH, 944 Chestnut Ridge Road, Morgantown, WV 26505, U.S.A.
• Federal Fiscal Year:
  1985
• Peer Reviewed:
  True
• Source Full Name:
  Biochimica et Biophysica Acta
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:58ef1ac4455a05a0f14ef847ca880960accc7cc7d9c6c20a648367c104aadc973414fc3d55f0159bb684ee788c430388334e5b8e114577a0d96e60a583d04b58
• Download URL:
  https://stacks.cdc.gov/view/cdc/185346/cdc_185346_DS1.pdf
• File Type:
  [PDF - 620.85 KB ]