Alterations in rat alveolar surfactant phospholipids and proteins induced by administration of chlorphentermine.

Details

• Personal Author:
  Bowman L ; Miles PR ; Reasor MJ ; Tucker J ; Wright JR
• Description:
  The effects of the anorectic drug chlorphentermine (461789) on the composition and stability of alveolar surfactant phospholipids and proteins were studied in Sprague-Dawley-rats. The animals were given daily injections of the drug for either 3 or 10 days. Another group of animals was injected with saline and the same amount of food was given to both groups. Control animals were fed ad lib. The animals were sacrificed, and their alveolar macrophages and alveolar type-II cells were isolated. The incorporation of radiolabeled palmitate, choline and glycerol into disaturated phosphatidylcholines (PC) in both cell types was measured. Total protein was determined, and the proteins were separated on a slab gel by electrophoresis and transferred to nitrocellulose paper. Immunostaining by antibodies to specific surfactant proteins was conducted on the nitrocellulose papers. The antigenic surfactant proteins were quantitated by rocket immunoelectrophoresis. The drug induced increases in surfactant total phospholipids, disaturated PC, and total protein in the lavage, and in surfactant apoprotein on the alveolar surface. Experiments were conducted with labeled substrates of disaturated PC, measuring their incorporation into disaturated PC and release into extracellular spaces. Based on these measurements, the authors conclude that the phospholipidosis does not seem to result from an increase in the synthesis of disaturated PC. The chlorphentermine induced phospholipidosis is believed to be due to decreased rates of phospholipid degradation. The drug may be bound to surfactant phospholipids, making them resistant to pulmonary phospholipases. [Description provided by NIOSH]
• Subjects:
  Immune System Laboratories Lipids Lung Occupational Health Respiratory System Safety
• Keywords:
  Alveolar-cells Author Keywords: Lung Surfactant Cell-metabolism Chlorphentermine Immunochemistry In-vivo-studies Laboratory-animals NIOSH-Author Phospholipid Pulmonary-clearance Pulmonary-function

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• ISSN:
  0006-3002
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  ALOSH - Appalachian Laboratory for Occupational Safety and Health
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  167-178
• Volume:
  877
• Issue:
  1
• NIOSHTIC Number:
  nn:00167928
• Citation:
  Biochim Biophys Acta 1986 Jun; 877(1):167-178
• Contact Point Address:
  P. R. Miles, Physiology Section, ALOSH, 944 Chestnut Ridge Road, Morgantown, WV 26505, U.S.A.
• CAS Registry Number:
  Chlorphentermine (CAS RN 461-78-9)
• Federal Fiscal Year:
  1986
• Peer Reviewed:
  True
• Source Full Name:
  Biochimica et Biophysica Acta
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:f3f8bed497e7c39d2a1ae403aea8ad72f9f8ce02a3e0202635bfa7762f91e2041d5224bb77c3568751300316ff0e316890fbb3741f6da6d7c113c7e3d5b08133
• Download URL:
  https://stacks.cdc.gov/view/cdc/185148/cdc_185148_DS1.pdf
• File Type:
  [PDF - 1.42 MB ]