2,5-Hexanedione alters microtubule assembly I. Testicular atrophy, not nervous system toxicity, correlates with enhanced tubulin polymerization.

Details

• Personal Author:
  Boekelheide K
• Description:
  The assembly and structural alterations in purified brain and testis tubulin of Charles-River-CD-rats fed either a 1 percent solution of 2,5-hexanedione (110134) (HD), or a 0.035 percent solution of 3,4- dimethyl-2,5-hexanedione (25234791) (DMHD), were analyzed. The animals were killed after 4 weeks of treatment. Brain and testis tubulins for in-vitro assembly experiments were purified using diethylaminoethyl-Sephacel. The weights of both treated groups were significantly less than those of the controls, and they both showed evidence of similar nervous system impairments. The testes of HD treated animals weighed less than those of the controls and showed several histological features indicating injury, while the testes of DMHD animals showed no significant differences to the controls. The tubulin content in brain and testis crude supernatants had not changed in either group of treated animals, compared to the controls. Purified brain and testis tubulins from HD treated rats assembled earlier and more rapidly than those from the control rats. Brain tubulins from DMHD treated rats assembled in a similar manner to those of the controls, while testis tubulin from these rats exhibited an assembly pattern intermediate to that of controls and HD treated animals. Immunoblotting with antitubulin antibodies indicated the presence of a covalently crosslinked tubulin in the brains of HD treated rats. The author concludes that microtubule assembly alterations may represent the biochemical mechanism of HD induced testicular atrophy, rather than the cause of nervous system disorders. [Description provided by NIOSH]
• Subjects:
  Biochemistry Butanones Histology Laboratories Nervous System Nervous System Disorders Occupational Health Safety Urogenital Diseases
• Keywords:
  Biochemical-analysis Cellular-structures Central-nervous-system-disorders In-vitro-studies In-vivo-studies Ketones Laboratory-animals Neurotoxicity NIOSH-Grant NIOSH-Publication Reproductive-system-disorders

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• ISSN:
  0041-008X
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 1 ; Rhode Island
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  370-382
• Volume:
  88
• Issue:
  3
• NIOSHTIC Number:
  nn:00171825
• Citation:
  Toxicol Appl Pharmacol 1987 May; 88(3):370-382
• Contact Point Address:
  Pathology and Lab Medicine Div. of Biology and Medicine Brown University, Box G Providence, RI 02912
• CAS Registry Number:
  2,5-Hexanedione (CAS RN 110-13-4) ; 3,4-Dimethyl-2,5-hexanedione (CAS RN 25234-79-1)
• Federal Fiscal Year:
  1987
• NORA Priority Area:
  Reproductive System Disorders
• Performing Organization:
  Brown University, Providence, Rhode Island
• Peer Reviewed:
  True
• Start Date:
  19850927
• Source Full Name:
  Toxicology and Applied Pharmacology
• End Date:
  19880831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:301a18b94ce7ed900dad323c3368d89dcd70d4ed3580299e7009d63d9cf00db12903b1578eb57b25ec7ae42000049f3a6eb6f2823612b6a71aedcf878c6d84aa
• Download URL:
  https://stacks.cdc.gov/view/cdc/185259/cdc_185259_DS1.pdf
• File Type:
  [PDF - 808.94 KB ]