Interaction between calcium and cadmium in the 1,25-dihydroxyvitamin D3 stimulated rat duodenum.

Details

• Personal Author:
  Foulkes EC ; Toraason M
• Description:
  The interaction between calcium (7440702) and cadmium (7440439) was investigated in rat duodenum. Male Sprague-Dawley-rats were maintained on a diet adequate in vitamin-D (1406162), calcium, and phosphorus (7723140); temperature, humidity and light were controlled. The vitamin-D metabolite 1,25-dihydroxyvitamin-D3 was administered intraperitoneally at 0.5 microgram per 50 microliters 4 hours prior to intestinal transport measurements; the control rats were given vehicle alone. The intestinal transport of calcium and cadmium was measured with everted duodenal sacs using appropriate biological solutions. Tritiated polyethylene-glycol was used as a non absorbable volume marker. For transport of calcium, labeled calcium-45 was employed; for cadmium, labeled cadmium-109 was employed. Unlabeled calcium or cadmium was included in duodenal mucosal bathing solution only when used to inhibit the transport of the other. Radionuclides were counted in a programmed scintillation spectrometer. The ratio of serosal to mucosal calcium concentration was initially maintained to be 1. Alterations of this ratio were evaluated experimentally. During the 60 minutes of incubation, calcium was actively transported and further enhanced by the vitamin- D metabolite; the serosal to mucosal ratio was 3.35. Cadmium at a 10 micromolar concentration abolished the stimulatory effect of the vitamin-D metabolite. Cadmium at a 100 micromolar dose completely blocked the active transport. Cadmium uptake and transport were not altered by the vitamin-D metabolite; cadmium accumulation in mucosal tissue was significantly inhibited by calcium at 1 millimolar concentration. The authors conclude that the interaction between calcium and cadmium in the duodenal mucosa is related to the action of cadmium in blocking active transport processes. [Description provided by NIOSH]
• Subjects:
  Animals Metals, Heavy Occupational Health Safety Toxicology
• Keywords:
  Animal-studies Biological-effects Cell-wall-permeability Drug-interaction Heavy-metals Medical-research NIOSH-Author Quantitative-analysis Radiochemical-analysis Transport-mechanisms
• ISSN:
  0041-008X
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DBBS - Division of Biomedical and Behavioral Sciences
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  98-104
• Volume:
  75
• Issue:
  1
• NIOSHTIC Number:
  nn:00146219
• Citation:
  Toxicol Appl Pharmacol 1984 Aug; 75(1):98-104
• CAS Registry Number:
  Cadmium (CAS RN 7440-43-9) ; Calcium (CAS RN 7440-70-2) ; Phosphorus (CAS RN 7723-14-0) ; Vitamin D (CAS RN 1406-16-2)
• Federal Fiscal Year:
  1984
• Peer Reviewed:
  True
• Source Full Name:
  Toxicology and Applied Pharmacology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:98151e922ec24317e8b8b9e3309308c0820fb334b690be6b4a769bd1f04d080206c746224a59e8b4122f1c9d4754d651153fdb500d37c258af28b2dbca90fd73
• Download URL:
  https://stacks.cdc.gov/view/cdc/184822/cdc_184822_DS1.pdf
• File Type:
  [PDF - 587.42 KB ]