Cutaneous metabolism of benzo[a]pyrene: comparative studies in C57BL/6N and DBA/2N mice and neonatal Sprague-Dawley rats.

Details

• Personal Author:
  Bickers DR ; Mukhtar H ; Yang SK
• Description:
  Benzo(a)pyrene (50328) (BaP) metabolism in skin microsomes were studied in rodents differing in inducibility of cutaneous enzyme activity. The backs of female C57BL/6N-mice responsive to aryl-hydrocarbon-hydroxylase (AHH) induction, female DBA/2N-mice not AHH responsive, and tumor resistant neonatal Sprague-Dawley-rats were painted with 100 milligrams per kilogram of the polychlorinated biphenyl (PCB) Aroclor-1254 (11097691) or the polycyclic aromatic hydrocarbon 3-methylcholanthrene (56495) (3-MC) in acetone or acetone only. Animals were sacrificed after 24 hours, and skin microsomes were obtained and incubated with BaP. AHH activity and formation of BaP metabolites were determined. Compared to controls, PCB increased AHH activity 16 to 18 times in C57-mice and rats, but only 2 times in DBA-mice, while 3-MC increased activity 1.6 to 4 times in mice and 11 times in rats. Total BaP metabolites in control DBA-mice were double those in other controls. PCB increased total BaP metabolite formation 10 to 12 times in C57-mice and rats and 3 times in DBA-mice, while 3-MC increased total metabolites 4 to 8 times in C57-mice and rats but did not affect DBA-mice. In C57-mice and rats, total phenols and the procarcinogen benzo(a)pyrene-7,8-diol (57303998) were increased 11 to 20 and 10 to 12 times, respectively, by PCB, and 7 to 8 and 4 to 7 times by 3-MC; values in DBA-mice ranged from 0.8 to 2.5 times control. The authors conclude that enzyme activities of skin microsomes and the quantity of BaP metabolites formed are not reliable indicators of skin tumor susceptibility to BaP. [Description provided by NIOSH]
• Subjects:
  Biphenyl Compounds Carcinogens Energy Metabolism Enzymes Hydrocarbons Infant, Newborn Laboratories Occupational Health Polycyclic Aromatic Hydrocarbons Safety Skin

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• Keywords:
  Author Keywords: Skin Benzo[a]pyrene Biphenyls Carcinogen Comparative Toxicology Enzyme Activity Genetic Factors High Performance Liquid Chromatography Laboratory Animals Metabolism Microsomal Enzymes Neonates Polycyclic Aromatic Hydrocarbons Skin Tumors

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• ISSN:
  0009-2797
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Maryland ; Ohio ; OSHA Region 3 ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  263-270
• Volume:
  43
• Issue:
  3
• NIOSHTIC Number:
  nn:00141512
• Citation:
  Chem-Biol Interact 1983 Mar; 43(3):263-270
• CAS Registry Number:
  3-Methylcholanthrene (CAS RN 56-49-5) ; Benzo[a]pyrene (CAS RN 50-32-8) ; Benzo[a]pyrene-7,8-diol (CAS RN 57303-99-8) ; PCB-1254 (CAS RN 11097-69-1)
• Federal Fiscal Year:
  1983
• Performing Organization:
  Case Western Reserve University, Cleveland, Ohio
• Peer Reviewed:
  True
• Start Date:
  19800929
• Source Full Name:
  Chemico-Biological Interactions
• End Date:
  19830831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:5a428174d8160da2990f9ad84cb693af2b8ce457738cc1e170b1f77389ca1fce4a988960f1c03057ac584594b074e1422217fe1e74d049bf90a7bd0f37a176d0
• Download URL:
  https://stacks.cdc.gov/view/cdc/184761/cdc_184761_DS1.pdf
• File Type:
  [PDF - 479.38 KB ]