Effects of hydroxyl compounds on the interaction of concanavalin A with polysaccharides.

Details

• Personal Author:
  Plow EF ; Resnick H
• Description:
  The effects of hydroxyl compounds on the binding of Concanavalin-A to polysaccharides were investigated. Mixtures of rabbit glycogen and Concanavalin-A were treated with varying amounts of 1 of 20 hydroxyl compounds for 10 minutes. The percent inhibition of Concanavalin-A binding to polysaccharides was calculated from the absorbance value read at 420 nanometers. The percent inhibition was correlated with the number of hydroxyl groups on the carbon skeleton, and with the number of carbon atoms. The concentrations required to produce 50 percent inhibition ranged from 0.000124 millimoles for methyl-alpha-D-glucoside (97303) to 12.80 millimoles for isopropanol (67630). Ethylene-glycol (107211) was a more potent inhibitor than 1,3-propanediol (57556), which in turn was more potent than 1,3-propanediol (504632). Both methanol (67561) and ethanol (64175) increased the effective binding of Concanavalin-A to polysaccharides. Increases in binding effectiveness with straight chain alcohols was related to chain length. The authors conclude that the binding forces between Concanavalin-A and polysaccharides are hydrophilic in nature and are dependent on hydrogen bonding. Conversion of a hydroxyl group to an aldehydic or carboxyl function further enhances the binding inhibition effect of the compound. [Description provided by NIOSH]
• Subjects:
  Animals Biochemistry Biological Assay Chemistry Occupational Health Safety Temperature
• Keywords:
  Alcohols Animal-studies Antibody Bioassays Biochemical-tests Chemical-binding Chemical-properties Proteins Temperature-measurement
• ISSN:
  0006-3002
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  ALFORD - Appalachian Laboratory for Occupational Respiratory Disease
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  221
• Issue:
  3
• NIOSHTIC Number:
  nn:00130971
• Citation:
  Biochim Biophys Acta 1970 Dec; 221(3):657-661
• Contact Point Address:
  Edward F. Plow, Appalachian Laboratory for Occupational Respiratory Diseases, Bureau of Occupational Safety and Health, U.S. Public Health Service, Department of Health, Education and Welfare, Morgantown, W.Va. 26505
• CAS Registry Number:
  (±)-Propylene glycol (CAS RN 57-55-6) ; 1,3-Propanediol (CAS RN 504-63-2) ; Ethanol (CAS RN 64-17-5) ; Ethylene glycol (CAS RN 107-21-1) ; Isopropanol (CAS RN 67-63-0) ; Methanol (CAS RN 67-56-1) ; Methyl α-D-glucopyranoside (CAS RN 97-30-3)
• Federal Fiscal Year:
  1971
• Peer Reviewed:
  True
• Source Full Name:
  Biochimica et Biophysica Acta
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:f02aee50a15ef041c5564ef425b19f0bd9cb060d1a8b78f1d3f4bf0aa5f641459ac1e53d7c97e625a765807ee4433cc9e57ee22a0be0a84d5f61af921d7e0f25
• Download URL:
  https://stacks.cdc.gov/view/cdc/184625/cdc_184625_DS1.pdf
• File Type:
  [PDF - 280.17 KB ]