Monitoring delayed-onset pulmonary hypersensitivity in guinea pigs.

Details

• Personal Author:
  Alarie Y ; Karol MH ; Stadler J ; Underhill D
• Description:
  The delayed onset pulmonary reaction to purified tuberculin protein (PPD) was monitored in guinea pigs. Three to five weeks after female English-smooth-haired-guinea-pigs were sensitized to 100 microliters of Freund's complete adjuvant and 3 to 5 weeks later they received intradermal injections of 50 microliters of PPD, and skin sensitivity was evaluated. Pulmonary response was evaluated after animals showing severe skin reactions were placed in plethysmographs connected to chambers containing concentration of 24 micrograms PPD per liter air flowing at 20 liters per minute for 48 hours. Skin reactions were positive in 11 of 28 animals 18 to 24 hours after exposure to PPD. Respiratory rates increased in all animals from 32 to 73 percent 9 to 12 hours after exposure. Lung sections from animals sacrificed 10, 24, and 48 hours after exposure consistently showed mononuclear cell infiltration of lymphocytes and thickened alveolar walls, with the most extensive changes occurring in the 48 hour sample. The authors conclude that this measurement system would be useful for assessing pulmonary sensitizing properties of industrial chemicals. [Description provided by NIOSH]
• Subjects:
  Health Care Facilities Workforce And Services Hypersensitivity Immune System Laboratories Lung Occupational Health Respiratory System Safety Toxicology
• Keywords:
  Allergic Reactions Analytical Methods Immunology Laboratory Animals Medical Monitoring Pulmonary System
• ISSN:
  0041-008X
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; Pennsylvania
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  277-285
• Volume:
  61
• Issue:
  2
• NIOSHTIC Number:
  nn:00121924
• Citation:
  Toxicol Appl Pharmacol 1981 Nov; 61(2):277-285
• Contact Point Address:
  Meryl H. Karol, Department of Industrial Environmental Health Sciences, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, Pennsylvania 15261 USA
• Federal Fiscal Year:
  1982
• Performing Organization:
  University of Pittsburgh
• Peer Reviewed:
  True
• Source Full Name:
  Toxicology and Applied Pharmacology
• End Date:
  19830430
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:1633e94f9c9e1622c150148eaafe18ae3fdecabd6973694fcd9c2f3120be4efcac08ebf5bae609a3bdee05b0861828537d45f0e58b42338ed4d7e35df7dba6dc
• Download URL:
  https://stacks.cdc.gov/view/cdc/184534/cdc_184534_DS1.pdf
• File Type:
  [PDF - 589.11 KB ]