Monitoring and Characterization of Oseltamivir-Resistant Pandemic (H1N1) 2009 Virus, Japan, 2009–2010

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Ujike, Makoto ; Ejima, Miho ; Anraku, Akane ; Shimabukuro, Kozue ; Obuchi, Masatsugu ; Kishida, Noriko ; Hong, Xu ; Takashita, Emi ; Fujisaki, Seiichiro ; Yamashita, Kazuyo ; Horikawa, Hiroshi ; Kato, Yumiko ; Oguchi, Akio ; Fujita, Nobuyuki ; Tashiro, Masato ; Odagiri, Takato
• Corporate Authors:
  Influenza Virus Surveillance Group of Japan
• Description:
  To monitor and characterize oseltamivir-resistant (OR) pandemic (H1N1) 2009 virus with the H275Y mutation, we analyzed 4,307 clinical specimens from Japan by neuraminidase (NA) sequencing or inhibition assay; 61 OR pandemic (H1N1) 2009 viruses were detected. NA inhibition assay and M2 sequencing indicated that OR pandemic (H1N1) 2009 virus was resistant to M2 inhibitors, but sensitive to zanamivir. Full-genome sequencing showed OR and oseltamivir-sensitive (OS) viruses had high sequence similarity, indicating that domestic OR virus was derived from OS pandemic (H1N1) 2009 virus. Hemagglutination inhibition test demonstrated that OR and OS pandemic (H1N1) 2009 viruses were antigenically similar to the A/California/7/2009 vaccine strain. Of 61 case-patients with OR viruses, 45 received oseltamivir as treatment, and 10 received it as prophylaxis, which suggests that most cases emerged sporadically from OS pandemic (H1N1) 2009, due to selective pressure. No evidence of sustained spread of OR pandemic (H1N1) 2009 was found in Japan; however, 2 suspected incidents of human-to-human transmission were reported.
• Subjects:
  Adolescent Adult Antiviral Agents Child Child, Preschool Drug Resistance, Viral Female Hemagglutination Inhibition Tests Humans Infant Infant, Newborn Influenza, Human Influenza A Virus, H1N1 Subtype Male Microbial Sensitivity Tests Middle Aged Mutation Neuraminidase Oseltamivir Pandemics Sequence Analysis, DNA Young Adult

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• Source:
  Emerg Infect Dis. 17(3):470-479.
• Document Type:
  Journal Article
• Place as Subject:
  Japan
• Location:
  Asia
• Volume:
  17
• Issue:
  3
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:df11f6a54db3ec81c9a6f58e435736e387c2f865393a906df388cb995fa34a2f
• Download URL:
  https://stacks.cdc.gov/view/cdc/18446/cdc_18446_DS1.pdf
• File Type:
  [PDF - 457.07 KB ]