In vitro activation of the alternative pathway of complement by settled grain dust.

Details

• Personal Author:
  Gladish ME ; Major, Pervis C. ; Mull JC ; Olenchock, Stephen A. ; Peach, Michael J. ; Taylor G
• Description:
  The effects of settled grain dust on the human serum complement cascade were investigated. Settled grain dust from several active grain elevators in the Superior Duluth areas of the United States was mixed with normal human serum. Aliquots were analyzed for factor-B conversion and hemolytic activity. Factor-B conversion also was tested after addition of various chelators. Complement consumption also was tested in dust treated sera from C4 deficient guinea pigs. Factor-B conversion and reductions in hemolytic complement consumption were dose related. Dust induced factor-B conversion was inhibited by ethylenediaminetetraacetate but not by ethylene-glycol-bis-(beta-amino-ethyl-ether)-N,N'-tetraacetic-acid. Complement reductions also were seen in the treated sera from C4 deficient guinea-pigs. The authors conclude that these samples of grain dust produce hemolytic consumption via the alternative pathway. They suggest that chronic low level exposure to aerosolized, biologically active grain dust could cause respiratory disorders among grain workers. [Description provided by NIOSH]
• Subjects:
  Air Pollutants, Occupational Biochemistry Dust Energy Metabolism Hematologic Tests Immune System Laboratories Occupational Health Particulate Matter Respiratory Tract Diseases Safety

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• Keywords:
  Airborne-dusts Dose-response Hematology Humans Immunology Laboratory-animals Metabolism NIOSH-Author Plants Pulmonary-system-disorders
• ISSN:
  0091-6749
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  295-300
• Volume:
  62
• Issue:
  5
• NIOSHTIC Number:
  nn:00116204
• Citation:
  J Allergy Clin Immunol 1978 Nov; 62(5):295-300
• Federal Fiscal Year:
  1979
• NORA Priority Area:
  Pulmonary System Disorders
• Peer Reviewed:
  True
• Source Full Name:
  The Journal of Allergy and Clinical Immunology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:034e061d543d68dfd4c8a27a7507ac29776526b8d04634e3bdc6140732a00c81e56993d59fc7a99bcec1e20f4b297b971cba72e660ce60a1ce57297bee0cd4c4
• Download URL:
  https://stacks.cdc.gov/view/cdc/184451/cdc_184451_DS1.pdf
• File Type:
  [PDF - 1.17 MB ]