Metabolism of nitrous oxide by human and rat intestinal contents.

Details

• Personal Author:
  Cohen EN ; Hong K ; O'Neil JR ; Trudell JR
• Description:
  The reduction of nitrous-oxide (10024972) (N2O) to nitrogen (7727379) (N2) by intestinal contents was studied. Sprague-Dawley- rats were treated with antibiotics and sacrificed. Intestines and contents were removed from treated and untreated rats. Homogenates of the contents of small and large intestines of rats, and of human large intestinal contents were prepared. Homogenates were also prepared from the intestinal wall of rats. Homogenates were degassed and treated with isotopically labeled N2O (15N20) and incubated. Homogenates were also treated with 5, 10, and 20 percent oxygen (2). The amount of 15N15N produced by homogenates indicated that intestinal contents from rats and humans metabolize N20. Metabolism was greatly inhibited by 02 at 10 and 20 percent, and partially inhibited at 5 percent O2. Intestinal wall homogenates had a very low rate of metabolism. The authors conclude that intestinal contents possess high N20 reductase activity under anaerobic conditions and moderate activity under low 02 tension normally found in intestines. They note that free radical intermediates may be produced from the metabolism of N20 to N2 which may be important to the etiology of carcinogenesis. [Description provided by NIOSH]
• Subjects:
  Biochemistry Digestive System Energy Metabolism Laboratories Nitrogen Oxides Occupational Health Safety
• Keywords:
  Gastrointestinal-system Laboratory-animals Metabolism NIOSH-Grant NIOSH-Publication Nitrogen-oxides
• ISSN:
  0003-3022
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  16-19
• Volume:
  52
• Issue:
  1
• NIOSHTIC Number:
  nn:00115386
• Citation:
  Anesthesiology 1980 Jan; 52(1):16-19
• Contact Point Address:
  Dr. Trudell, Associate Professor of Chemistry in Anesthesia, Stanford University 300 Pasteur Drive, Stanford, Calif 94305
• CAS Registry Number:
  Nitrogen (CAS RN 7727-37-9) ; Nitrous oxide (CAS RN 10024-97-2)
• Federal Fiscal Year:
  1980
• Performing Organization:
  Stanford University, Stanford, California
• Peer Reviewed:
  True
• Start Date:
  19770601
• Source Full Name:
  Anesthesiology
• End Date:
  19801231
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:0613d8b5e275692f1be128a908abec1ea5aebb81185c18485dd32fc93f3bff4f29c956146526e712550ea9d25bdf68cfe394fe084be1d2f67ee0fd8822f1a33e
• Download URL:
  https://stacks.cdc.gov/view/cdc/184437/cdc_184437_DS1.pdf
• File Type:
  [PDF - 2.90 MB ]