Precipitation of serum proteins by extracts of cotton dust and stems: identification of β-lipoprotein and production of specific antibodies.

Details

• Personal Author:
  Kutz SA ; Major, Pervis C. ; Mentnech MS ; Olenchock, Stephen A.
• Description:
  Serum proteins reacting with a pseudoimmune precipitating agent found in cardroom cotton dust and cotton stem material were identified. Specimen particles were ground and extracted separately at 4 degrees C and the supernatant fluids were sterilized and concentrated 40 fold. Antisera to cotton bracts and leaves were developed in rabbits. Human and rabbit sera were examined for immune and pseudoimmune precipitation, and sera from normal human subjects, cotton textile workers and a serum preparation lacking beta-lipoprotein were separately mixed with either the cotton dust or stem extract, incubated and centrifuged. No true precipitating antibodies to extracts of cotton bracts, carpels, stems, leaves, immature cotton lint, or cardroom cotton dust were found in the 59 human sera samples. Precipitating antibodies were produced in the rabbit immunization test. The authors note that the plant agent responsible for precipitation of the beta-lipoprotein and gamma globulin was suspected to be a polyphenic tannin. [Description provided by NIOSH]
• Subjects:
  Dust Hematologic Tests Hypersensitivity Immune System Occupational Health Safety Textile Industry
• Keywords:
  Allergic-reactions Cotton-dusts Hematology Immunology NIOSH-Author Textiles
• ISSN:
  0013-9351
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  476-484
• Volume:
  22
• Issue:
  2
• NIOSHTIC Number:
  nn:00112381
• Citation:
  Environ Res 1980 Aug; 22(2):476-484
• Federal Fiscal Year:
  1980
• Peer Reviewed:
  True
• Source Full Name:
  Environmental Research
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:48fd92b19c25a7107ff9a2c55ee20d1bc87716a1c798304ef95a182d1d44b50c32f53c658f4085e57fa7901102dd00ea3bfd6b6c128ebce6e33b27fa2159d3aa
• Download URL:
  https://stacks.cdc.gov/view/cdc/184404/cdc_184404_DS1.pdf
• File Type:
  [PDF - 4.15 MB ]