Multicellular in vivo sister-chromatid exchanges induced by urethane.

Details

• Personal Author:
  Alarie Y ; Cheng M ; Conner MK
• Description:
  Multicellular in-vivo sister chromatid exchanges (SCE) induced by urethane (51796) were studies in male BDF-1-mice. Partially hepatectomized and intact mice were exposed for 4 hours on day 5 post hepatectomy to 0.035, 0.11, 0.22, 0.475, 0.90, or 2.06 milligrams per liter (mg/l) urethane (51796). Mice were also given intravenous infusions for 4 hours or a single dose intraperitoneally (ip) of 193mg per kilogram urethane prior to 5-bromo-2'-deoxyuridine (59143) (BrdU) incorporation testing. On day 6 post hepatectomy, mice were given an ip injection of 100 micrograms of colchicine and were sacrificed 4 hours later. Clear dose relationships were apparent in all cell types in both hepatectomized and intact mice. No significant increase in SCE frequencies were observed at 0.035mg/l, but at doses of 0.1mg/l and greater, induced SCE frequencies were linearly related to urethane concentrations. No significant differences in intact and hepatectomized mice were noted. Bone marrow response was significantly lower than that of alveolar macrophages. Inhalation and intravenous infusions of the same total dose of urethane produced comparable SCE responses, but a single ip injection produced significantly higher SCE frequencies in bone marrow and alveolar macrophages. The authors conclude that impaired liver function does not affect SCE frequencies. [Description provided by NIOSH]
• Subjects:
  Chromosomes Laboratories Mutagens Occupational Health Safety
• Keywords:
  Cellular Reactions Chromosome Damage Dose Response Genetic Disorders Invivo Study Laboratory Animals Liver Function Mutagenicity
• ISSN:
  0027-5107
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; Pennsylvania
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  223-231
• Volume:
  88
• Issue:
  2
• NIOSHTIC Number:
  nn:00106474
• Citation:
  Mutat Res 1981 Feb; 88(2):223-231
• Contact Point Address:
  Occupational Health University of Pittsburgh 130 DE Soto Street Pittsburgh, PA 15213
• CAS Registry Number:
  5-Bromo-2′-deoxyuridine (CAS RN 59-14-3) ; Carbamic acid, ethyl ester (CAS RN 51-79-6)
• Federal Fiscal Year:
  1981
• Performing Organization:
  University of Pittsburgh, Pittsburgh, Pennsylvania
• Peer Reviewed:
  True
• Start Date:
  19711001
• Source Full Name:
  Mutation Research
• End Date:
  19860531
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:6025ba1eda45779c316d144cf0f326fb3cca50d7812831d987de5beb0e45cc7855103bd622444f2892ccc61a39254df4bf638574c5d8b300a83282c68653de44
• Download URL:
  https://stacks.cdc.gov/view/cdc/184382/cdc_184382_DS1.pdf
• File Type:
  [PDF - 494.95 KB ]