Extracts of airborne grain dusts activate alternative and classical complement pathways.

Details

• Personal Author:
  Major, Pervis C. ; Mull JC ; Olenchock, Stephen A.
• Description:
  The action of airborne grain dust extracts on the alternative and classical complement pathways, in humans was studied. Airborne grain dust extracts were characterized in terms of protein nitrogen, and gram negative bacterial endotoxic contamination. Extracts were added to 0.5 milliliters of pooled normal human serum, and the aliquots were tested for complement activities, C3 conversion in chelated NHS, and chemotaxis of polymorphonuclear leukocytes. Extracts consumed hemolytic complement in a dose dependent manner. No relationship was determined between complement activity and either protein or endotoxin concentrations. Differential serum chelation with grain extracts proved that the alternative pathways were activated, while hemolytic titers of the early complement components indicated that the classical pathway was involved. Complement was required for extract treated sera, which was chemotactic for PMN. The authors conclude that extracts of airborne grain dusts can activate alternative and classical complement pathways in-vitro, and can generate complement dependent directional migration of human PMN. [Description provided by NIOSH]
• Subjects:
  Air Pollutants, Occupational Bacteria Cytology Dust Hematologic Tests Occupational Health Particulate Matter Respiratory Tract Diseases Safety
• Keywords:
  Airborne-dusts Analytical-methods Bacterial-dusts Hematology In-vitro-studies NIOSH-Author Pulmonary-system-disorders
• ISSN:
  0003-4738
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  23-28
• Volume:
  44
• Issue:
  1
• NIOSHTIC Number:
  nn:00092666
• Citation:
  Ann Allergy 1980 Jan; 44(1):23-28
• Federal Fiscal Year:
  1980
• NORA Priority Area:
  Pulmonary System Disorders
• Peer Reviewed:
  True
• Source Full Name:
  Annals of Allergy
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:d6519b09ea61529da2c25eceed570d8a3d3481ad8797865140fdb44df2d8549d05ceacbc846505969f59c762452ffe689717eef39ec2a06988efd001759251ba
• Download URL:
  https://stacks.cdc.gov/view/cdc/184303/cdc_184303_DS1.pdf
• File Type:
  [PDF - 789.64 KB ]