Details:

Alternative Title:
Emerg Infect Dis
Personal Author:
Engelthaler, David M. ; Chiller, Tom ; Schupp, James A. ; Colvin, Joshua ; Beckstrom-Sternberg, Stephen M. ; ... More +
Engelthaler, David M. ; Chiller, Tom ; Schupp, James A. ; Colvin, Joshua ; Beckstrom-Sternberg, Stephen M. ; Driebe, Elizabeth M. ; Moses, Tracy ; Tembe, Waibhav ; Sinari, Shripad ; Beckstrom-Sternberg, James S. ; Christoforides, Alexis ; Pearson, John V. ; Carpten, John ; Keim, Paul ; Peterson, Ashley ; Terashita, Dawn ; Balajee, S. Arunmozhi Less -
Description:
Next-generation sequencing enables use of whole-genome sequence typing (WGST) as a viable and discriminatory tool for genotyping and molecular epidemiologic analysis. We used WGST to confirm the linkage of a cluster of Coccidioides immitis isolates from 3 patients who received organ transplants from a single donor who later had positive test results for coccidioidomycosis. Isolates from the 3 patients were nearly genetically identical (a total of 3 single-nucleotide polymorphisms identified among them), thereby demonstrating direct descent of the 3 isolates from an original isolate. We used WGST to demonstrate the genotypic relatedness of C. immitis isolates that were also epidemiologically linked. Thus, WGST offers unique benefits to public health for investigation of clusters considered to be linked to a single source.
Subject:
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Cluster Analysis
Coccidioides
Coccidioidomycosis
DNA, Fungal
Fungi
Genome, Fungal
Genotype
Genotyping
Humans
Molecular Epidemiology
Mycological Typing Techniques
Next Generation Sequencing
Organ Transplantation
Phylogeny
Polymorphism, Single Nucleotide
Research
Sequence Alignment
Sequence Analysis, DNA
Species Specificity
Whole Genome Sequence Typing
Molecular Epidemiology

Source:
Emerg Infect Dis. 17(2):227-232.
Document Type:
Journal Article
Collection(s):
Emerging Infectious Diseases
Main Document Checksum:
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urn:sha256:8b86ad329590534611f3cb22d60f95f92b4cabfe8df7ae076a1b2072dcacb3c0
File Type:

Supporting Files

• 	license.txt	txt
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