Next-Generation Sequencing of Coccidioides immitis Isolated during Cluster Investigation

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Engelthaler, David M. ; Chiller, Tom ; Schupp, James A. ; Colvin, Joshua ; Beckstrom-Sternberg, Stephen M. ; Driebe, Elizabeth M. ; Moses, Tracy ; Tembe, Waibhav ; Sinari, Shripad ; Beckstrom-Sternberg, James S. ; Christoforides, Alexis ; Pearson, John V. ; Carpten, John ; Keim, Paul ; Peterson, Ashley ; Terashita, Dawn ; Balajee, S. Arunmozhi
• Description:
  Next-generation sequencing enables use of whole-genome sequence typing (WGST) as a viable and discriminatory tool for genotyping and molecular epidemiologic analysis. We used WGST to confirm the linkage of a cluster of Coccidioides immitis isolates from 3 patients who received organ transplants from a single donor who later had positive test results for coccidioidomycosis. Isolates from the 3 patients were nearly genetically identical (a total of 3 single-nucleotide polymorphisms identified among them), thereby demonstrating direct descent of the 3 isolates from an original isolate. We used WGST to demonstrate the genotypic relatedness of C. immitis isolates that were also epidemiologically linked. Thus, WGST offers unique benefits to public health for investigation of clusters considered to be linked to a single source.
• Subjects:
  Cluster Analysis Coccidioides Coccidioidomycosis DNA, Fungal Fungi Genome, Fungal Genotype Genotyping Humans Molecular Epidemiology Mycological Typing Techniques Next Generation Sequencing Organ Transplantation Phylogeny Polymorphism, Single Nucleotide Research Sequence Alignment Sequence Analysis, DNA Species Specificity Whole Genome Sequence Typing

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• Source:
  Emerg Infect Dis. 17(2):227-232.
• Document Type:
  Journal Article
• Volume:
  17
• Issue:
  2
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:8b86ad329590534611f3cb22d60f95f92b4cabfe8df7ae076a1b2072dcacb3c0
• Download URL:
  https://stacks.cdc.gov/view/cdc/18337/cdc_18337_DS1.pdf
• File Type:
  [PDF - 703.50 KB ]