Outbreak of Corynebacterium pseudodiphtheriticum Infection in Cystic Fibrosis Patients, France

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Bittar, Fadi ; Cassagne, Carole ; Bosdure, Emmanuelle ; Stremler, Nathalie ; Dubus, Jean-Christophe ; Sarles, Jacques ; Reynaud-Gaubert, Martine ; Raoult, Didier ; Rolain, Jean-Marc
• Description:
  An increasing body of evidence indicates that nondiphtheria corynebacteria may be responsible for respiratory tract infections. We report an outbreak of Corynebacterium pseudodiphtheriticum infection in children with cystic fibrosis (CF). To identify 18 C. pseudodiphtheriticum strains isolated from 13 French children with CF, we used molecular methods (partial rpoB gene sequencing) and matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry. Clinical symptoms were exhibited by 10 children (76.9%), including cough, rhinitis, and lung exacerbations. The results of MALDI-TOF identification matched perfectly with those obtained from molecular identification. Retrospective analysis of sputum specimens by using specific real-time PCR showed that approximately 20% of children with CF were colonized with these bacteria, whereas children who did not have CF had negative test results. Our study reemphasizes the conclusion that correctly identifying bacteria at the species level facilitates detection of an outbreak of new or emerging infections in humans.
• Subjects:
  Adolescent Bacteria Bacterial Proteins Child Child, Preschool Children Corynebacterium Corynebacterium Infections Corynebacterium Pseudodiphtheriticum Cough Cystic Fibrosis Disease Outbreaks DNA, Bacterial Female France Humans Infant Intact Cell Mass Spectrometry Maldi-Tof Male Phylogeny Polymerase Chain Reaction Research Respiratory Infections Retrospective Studies Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Sputum

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• Source:
  Emerg Infect Dis. 16(8):1231-1236.
• Document Type:
  Journal Article
• Volume:
  16
• Issue:
  8
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:b832ad194551308786d9769f70865dfaba97e922c6d06feba7b8ac65259f7c11
• Download URL:
  https://stacks.cdc.gov/view/cdc/18245/cdc_18245_DS1.pdf
• File Type:
  [PDF - 252.93 KB ]