Development of an immunoassay method for the detection of alachlor in agricultural workers' urine: correlation with a gas chromatographic (GC) chemical method.

Details

• Personal Author:
  Biagini, Raymond E.
• Description:
  A NIOSH field study of commercial pesticide applicators was conducted to characterize their exposure to alachlor, a major component of Lasso, which is a commonly used pre-emergent herbicide. To assess exposure and estimate uptake of alachlor, worker urine samples were analyzed using two different techniques for alachlor or its metabolites. The first was a published gas chromatographic method for analysis of urinary alachlor metabolites. We also developed an enzyme-linked irnmunosorbent assay (ELISA) method. The ELISA method is based on the use of antibodies directed against alachlor. The antibodies are also tagged with an enzyme whose reaction products are colored; adding the substrate of the enzyme to the reaction mixture yields varying intensities of color which are related to alachlor concentration. Preliminary results indicate a high correlation between the two methods. Immunochemical technology appears to be a viable alternative to traditional wet chemical and instrumental methods of analyses for assessing exposures in agricultural environments. Benefits of this technology include simpler sample preparation, reduced cost and analysis time, and the potential for onsite field measurements. A detailed description of the ELISA method and the results of the analyses for the field study samples will be presented.
• Subjects:
  Accidents, Occupational Butanones Chemistry Hazardous Substances Occupational Health Safety
• Keywords:
  Agricultural Industry Agricultural Workers Alachlor Animals Antibodies Blood Serum ELISA Environmental Exposure Enzyme-linked Immunosorbent Assay Enzymes Exposure Levels Gas Chromatography Hazards Herbicides Humans Men Metabolites Pesticides Risk Factors Safety Practices Toxic Effects Women Work Environment Worker Health Workers

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• Publisher:
  National Institute for Occupational Safety and Health
• Document Type:
  Text
• Genre:
  Abstract
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DBBS - Division of Biomedical and Behavioral Sciences
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  550
• NIOSHTIC Number:
  nn:20055984
• Citation:
  Papers and Proceedings of the Surgeon General's Conference on Agricultural Safety and Health (FarmSafe 2000). Myers ML, Herrick RF, Olenchock SA, Myers JR, Parker, John E., Hard DL, Wilson K, eds. Atlanta, GA: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control, National Institute for Occupational Safety and Health, DHHS (NIOSH) Publication No. 92-105, 1992 Sep; :550
• Editor(s):
  Myers ML ; Herrick RF ; Olenchock SA ; Myers JR ; Parker, John E. ; Hard DL ; Wilson K
• Federal Fiscal Year:
  1992
• Peer Reviewed:
  False
• Source Full Name:
  Papers and Proceedings of the Surgeon General's Conference on Agricultural Safety and Health (FarmSafe 2000), April 30-May 3, 1991, Des Moines, Iowa
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:48b06d3e4c9b4b5b11bcf1bf3a08adfc1475f62bcc3d09a6fe8269e762b9de6e0522733084735e5e48e4628cea4b980d7f7908e4c305a28e9cb83e699c571d3f
• Download URL:
  https://stacks.cdc.gov/view/cdc/181756/cdc_181756_DS1.pdf
• File Type:
  [PDF - 71.63 KB ]