Raman spectroscopic studies on the mechanisms of membrane damage induced by quartz and the protective effect of aluminum citrate.

Details

• Personal Author:
  Cao CJ ; Lin KC ; Liu SJ
• Description:
  The molecular mechanisms of quartz (14808607) induced membrane damage were studied by Raman spectroscopy. The mechanisms for the protective effect of aluminum-citrate (31142560) were also examined. Liposomes prepared from dipalmitoyl-phosphatidylcholine (DPPC) were pretreated with aluminum-citrate or aluminum-chloride (7446700) or untreated. They were then treated with 0, 0.5, or 1.0 milligram quartz or titanium-dioxide (13463677) dust. Raman spectra were recorded. The extent of membrane damage was assessed by monitoring changes in the DPPC spectra. Quartz shifted the carbon/nitrogen stretching band located at 715 reciprocal centimeters (/cm) approximately 2 to 3/cm toward lower frequency and significantly decreased its intensity. The band width was significantly increased. The intensity ratios of bands in the 1000 to 1200/cm region, which represented carbon/carbon and phosphorus/oxygen skeletal stretching vibrations, were significantly decreased by quartz. Carbon/hydrogen stretching vibrations occurring in the 2800 to 2900/cm region were also decreased. These changes were largely eliminated by aluminum-citrate or aluminum-chloride pretreatment. Titanium-dioxide did not significantly affect any of the spectral bands. The authors conclude that quartz damages DPPC liposomes primarily by acting on the carbon/nitrogen bond in the choline group. This results in the liposomes being deformed to fit the globular surface of the quartz particles. The protective effect of aluminum-citrate or aluminum-chloride is due to a direct action on the surface of the quartz particles which prevents the interaction between the quartz surface and the liposome membrane. Titanium- dioxide exerts only a weak effect on liposome membranes.
• Subjects:
  • Accidents, Occupational
  • Occupational Health
  • Safety
  • Staff Development
• Keywords:
  • In vitro studies
  • Silica dusts
  • Mineral dusts
  • Aluminum compounds
  • Chemical structure
  • Membrane dysfunction
  • Phospholipids
  • Prophylaxis
  • Spectrographic analysis
  • Surface properties
• Document Type:
  Text
• Genre:
  Conference/Symposia Proceedings
• Place as Subject:
  OSHA Region 3 ; Pennsylvania
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  1181-1185
• NIOSHTIC Number:
  nn:00198764
• Citation:
  Proceedings of the VIIth International Pneumoconioses Conference, August 23-26, 1988, Pittsburgh, Pennsylvania, USA. Atlanta, GA: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control, National Institute for Occupational Safety and Health, DHHS (NIOSH) Publication No. 90-108, 1990 Nov; (Part II):1181-1185
• CAS Registry Number:
  • Aluminum chloride (CAS RN 7446-70-0)
  • Aluminum citrate (CAS RN 31142-56-0)
  • Quartz (SiO2) (CAS RN 14808-60-7)
  • Titania (CAS RN 13463-67-7)
• Federal Fiscal Year:
  1991
• Peer Reviewed:
  False
• Part Number:
  II
• Source Full Name:
  Proceedings of the VIIth International Pneumoconioses Conference, August 23-26, 1988, Pittsburgh, Pennsylvania, USA
• Download URL:
  https://stacks.cdc.gov/view/cdc/180639/cdc_180639_DS1.pdf
• File Type:
  [PDF - 166.93 KB]
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:48544c1ddd3a3bd8fd9f89393cc6db0f4ae7c87f7cd54d3570def0a51108175937b0dd36ea8522c121a6192fdedb23ad095f7a02ff028a7703ca5b117594e33a