Blood Meal Analysis to Identify Reservoir Hosts for Amblyomma Americanum Ticks

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Allan, Brian F. ; Goessling, Lisa S. ; Storch, Gregory A. ; Thach, Robert E.
• Description:
  Efforts to identify wildlife reservoirs for tick-borne pathogens are frequently limited by poor understanding of tick-host interactions and potentially transient infectivity of hosts under natural conditions. To identify reservoir hosts for lone star tick (Amblyomma americanum)-associated pathogens, we used a novel technology. In field-collected ticks, we used PCR to amplify a portion of the 18S rRNA gene in remnant blood meal DNA. Reverse line blot hybridization with host-specific probes was then used to subsequently detect and identify amplified DNA. Although several other taxa of wildlife hosts contribute to tick infection rates, our results confirm that the white-tailed deer (Odocoileus virginianus) is a reservoir host for several A. americanum-associated pathogens. Identification of host blood meal frequency and reservoir competence can help in determining human infection rates caused by these pathogens.
• Subjects:
  Amblyomma Americanum Animals Animals, Wild Bacteria Blood Borrelia Deer Disease Reservoirs DNA, Bacterial DNA Probes Ehrlichia Feeding Behavior Host-Pathogen Interactions Host Blood Meal Identification Humans Ixodidae Nucleic Acid Hybridization Polymerase Chain Reaction Research Reservoir Capacity RNA, Ribosomal, 16S RNA, Ribosomal, 18S Sequence Analysis, DNA Species Specificity Tick Infestations Ticks Vector-borne Infections White-tailed Deer Zoonoses

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• Source:
  Emerg Infect Dis. 16(3):433-440.
• Document Type:
  Journal Article
• Volume:
  16
• Issue:
  3
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:5c5829dda5693cf790eb788453f250338195a7415198c9437f88395d879a63a8
• Download URL:
  https://stacks.cdc.gov/view/cdc/17993/cdc_17993_DS1.pdf
• File Type:
  [PDF - 172.72 KB ]