Evaluation of the transformation assay using C3H 10T1/2 cells for use in screening chemicals for carcinogenic potential.

Details

• Personal Author:
  Cameron TP
• Description:
  An in-vitro transformation assay for screening the carcinogenic potential of chemicals was evaluated. C3H 10T 1/2 cells were cultured that had a plating efficiency of 23 percent, a saturation density of 590,000, and a generation time of about 16 hours. The assay was evaluated with and without an exogenous metabolic activation system using known chemical carcinogens by scoring for Type III foci and by determining the ability of cells from Type III foci to grow in soft agar and produce tumors when injected into irradiated C3H mice. Benzo(a)pyrene (50328) (BaP), 3- methylcholanthrene (56495) (3-MC), dibenz(a,h)anthracene (53703), and 7,12-dimethylbenz(a)anthracene (57976) gave positive responses without metabolic activation, but transformation frequency and dose at which a positive response occurred varied. Anthracene (120127), phenanthrene (85018), 2-acetylaminofluorene (53963) (2-AAF), and N- methyl-N-nitro-N-nitrosoguanidine (70257) (MNNG) were not active in the absence of metabolic activation. BaP was active in the presence of an S-9 activation system, but 3-MC and diethylnitrosamine (55185) (DEN) were not. An amplification modification caused MNNG to be active but did not increase the transformation caused by 3-MC over that occuring in the standard assay. The author concludes that the assay warrants further development to identify factors influencing the assay and determine procedures leading to standardization.
• Subjects:
  Accidents, Occupational Air Pollutants, Occupational Construction Materials Dust Environmental Monitoring Minerals Occupational Health Particulate Matter Preceptorship Safety
• Keywords:
  Bioassays Biochemical-tests Biotransformation Carcinogenesis Carcinogens In-vitro-study Medical-screening Screening-methods
• Publisher:
  NCI; EPA; NIOSH
• Document Type:
  Text
• Genre:
  Conference/Symposia Proceedings
• Place as Subject:
  District of Columbia ; Maryland ; OSHA Region 3
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  298-305
• NIOSHTIC Number:
  nn:00123092
• Citation:
  Proceedings of the first NCI/EPA/NIOSH collaborative workshop: progress on joint environmental and occupational cancer studies, May 6-8, 1980, Rockville, Maryland. Kraybill HF, Blackwood IC, Freas NB, eds. Washington, DC: National Cancer Institute, Environmental Protection Agency, National Institute for Occupational Safety and Health, 1980 May; :298-305
• CAS Registry Number:
  Benzo[a]pyrene (CAS RN 50-32-8) ; 3-Methylcholanthrene (CAS RN 56-49-5) ; Dibenz[a,h]anthracene (CAS RN 53-70-3) ; 7,12-Dimethylbenz[a]anthracene (CAS RN 57-97-6) ; Anthracene (CAS RN 120-12-7) ; Phenanthrene (CAS RN 85-01-8) ; AAF (CAS RN 53-96-3) ; N-Methyl-N′-nitro-N-nitrosoguanidine (CAS RN 70-25-7) ; Diethylnitrosamine (CAS RN 55-18-5)
• Editor(s):
  Kraybill HF ; Blackwood IC ; Freas NB
• Federal Fiscal Year:
  1980
• Peer Reviewed:
  False
• Source Full Name:
  Proceedings of the first NCI/EPA/NIOSH collaborative workshop: progress on joint environmental and occupational cancer studies, May 6-8, 1980, Rockville, Maryland
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:21dbed359512f10b6726a14c5f027802bed1592c3a4cc1303f3fe5a521b0e7bef8b7ee0b362288d4dfd1e9dd709e5ec2846bbc84a11687fd75aaef267138e179
• Download URL:
  https://stacks.cdc.gov/view/cdc/179467/cdc_179467_DS1.pdf
• File Type:
  [PDF - 317.62 KB ]