Cytogenetic evaluation of bone marrow cells in the rat following long-term inhalation exposure to nitrous oxide plus halothane.

Details

• Corporate Authors:
  National Institute for Occupational Safety and Health
• Description:
  The mutagenic potential of long-term exposure of 120 male Sprague- Dawley rats to nitrous-oxide (10024972) is measured by observing aberrations in bone marrow cell chromosomes. Forty rats serve as controls, 40 are exposed to 50ppm nitrous-oxide plus 1ppm halothane (151677), and 40 are exposed to 500ppm nitrous-oxide plus 10ppm halothane, for 7 hours a day, 5 days a week for 52 weeks. The most frequently found aberration is the chromatid gap. There is a significant increase in the number of gaps and this increase is dose- related. Chromatid breaks are the next most frequent aberrations observed. Chromosomal breaks are comparable among all groups. Chromosomal markers are significantly increased in the group receiving 500ppm nitrous-oxide. The incidence of polyploidy is comparable among control and treated groups. Fifty-nine percent of the animals in the first treatment group show aberrant cells; 68 percent in the second treatment group. It is concluded that exposure to 500ppm nitrous-oxide plus 10ppm halothane can cause chromosomal damage to bone marrow cells in the rat. (Contract No. 99-74-0046)
• Subjects:
  Accidents, Occupational Construction Industry Ergonomics Musculoskeletal Diseases Occupational Health Safety Staff Development
• Keywords:
  Anesthetics Chromosome-damage Contract-099-74-0046 Drugs Genetic-disorders Inhalation Mutagens NIOSH-Publication Toxic-gases Toxic-substances Toxicity

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• Document Type:
  Text
• Funding:
  Contract
• Genre:
  Final Contract Report
• Place as Subject:
  OSHA Region 3 ; Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  1-24
• NIOSHTIC Number:
  nn:00068605
• Citation:
  NIOSH 1976 Jan; :1-24
• CAS Registry Number:
  Nitrous oxide (CAS RN 10024-97-2) ; (±)-Halothane (CAS RN 151-67-7)
• Federal Fiscal Year:
  1976
• Performing Organization:
  Hazleton Laboratories America, Inc.
• Peer Reviewed:
  False
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:c4e723bc75a3b948c7e88f551d533fd38a04df8682fac1cbc6e03f8b1e2a1cf4180b736d32873bf6cfb8ae6afadf0c2df1ddf4ae9e98badd5b4f0cb22c22a44c
• Download URL:
  https://stacks.cdc.gov/view/cdc/178779/cdc_178779_DS1.pdf
• File Type:
  [PDF - 676.83 KB ]